Publication: Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro
3
Accepted Date
2010-11-30
Issued Date
2010-11-10
Copyright Date
2010
Resource Type
Language
eng
ISSN
1471-2180 (electronic)
Rights
Mahidol University
Rights Holder(s)
BioMed Central
Bibliographic Citation
Tandhavanant S, Thanwisai A, Limmathurotsakul D, Korbsrisate S, Day NP, Peacock SJ, et al. Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro. BMC Microbiol. 2010 Nov 30;10:303.
Suggested Citation
Sarunporn Tandhavanant, ศรัณย์พร ตัณฑวนันท์, Aunchalee Thanwisai, อัญชลี ฐานวิสัย, Direk Limmathurotsakul, ดิเรก ลิ้มมธุรสกุล, Sunee Korbsrisate, Day, Nicholas P.J., Peacock, Sharon J., Narisara Chantratita, นริศรา จันทรานิตย Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro. Tandhavanant S, Thanwisai A, Limmathurotsakul D, Korbsrisate S, Day NP, Peacock SJ, et al. Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro. BMC Microbiol. 2010 Nov 30;10:303.. doi:10.1186/1471-2180-10-303 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/724
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Title
Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro
Corresponding Author(s)
Other Contributor(s)
Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit
Mahidol University. Faculty of Tropical Medicine. Department of Immunology
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene
Mahidol University. Faculty of Medicine Siriraj Hospital. Department of Immunology.
Mahidol University. Faculty of Tropical Medicine. Department of Immunology
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene
Mahidol University. Faculty of Medicine Siriraj Hospital. Department of Immunology.
Abstract
BACKGROUND: Primary diagnostic cultures from patients with melioidosis
demonstrate variation in colony morphology of the causative organism,
Burkholderia pseudomallei. Variable morphology is associated with changes in the
expression of a range of putative virulence factors. This study investigated the
effect of B. pseudomallei colony variation on survival in the human macrophage
cell line U937 and under laboratory conditions simulating conditions within the
macrophage milieu. Isogenic colony morphology types II and III were generated
from 5 parental type I B. pseudomallei isolates using nutritional limitation.
Survival of types II and III were compared with type I for all assays.
RESULTS: Morphotype was associated with survival in the presence of H2O2 and
antimicrobial peptide LL-37, but not with susceptibility to acid, acidified
sodium nitrite, or resistance to lysozyme, lactoferrin, human neutrophil
peptide-1 or human beta defensin-2. Incubation under anaerobic conditions was a
strong driver for switching of type III to an alternative morphotype. Differences
were noted in the survival and replication of the three types following uptake by
human macrophages, but marked strain-to strain-variability was observed. Uptake
of type III alone was associated with colony morphology switching.
CONCLUSIONS: Morphotype is associated with phenotypes that alter the ability of
B. pseudomallei to survive in adverse environmental conditions.