Publication: Thai national reference preparation in blood coagulation. IV. Home made reference plasma for von Willebrand's factor assay.
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Issued Date
1993-10-01
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01252208
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2-s2.0-0027676667
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.76, No.10 (1993), 570-575
Suggested Citation
P. Talalak, P. Luenee, N. Opartkiattikul, V. Sangtawisin Thai national reference preparation in blood coagulation. IV. Home made reference plasma for von Willebrand's factor assay.. Journal of the Medical Association of Thailand. Vol.76, No.10 (1993), 570-575. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22687
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Title
Thai national reference preparation in blood coagulation. IV. Home made reference plasma for von Willebrand's factor assay.
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Abstract
Two lots of home made reference plasma: FVIII R:Ag 30/8/88 and FVIII R:Ag 18/10/88 were prepared by lyophilization of pooled normal human plasma. Modification of J. Cejka's technique was used to determine FVIII R:Ag. This technique was tested for reliability i.e. precision, reproducibility and sensitivity. The concentration of FVIII R:Ag, determined by calibration against the 1st British Standard for FVIII R:Ag, human 66/355, which was established by National Institute for Biological Standard and Control (NIBS & C), London, the WHO International Laboratory for Biological Standard, were respectively 1.058 and 1.023 Ag units/ml for FVIII R:Ag 30/8/88 and FVIII R:Ag 18/10/88, respectively. The precision of the procedure and the accuracy of FVIII R:Ag concentration of both lots were verified by using them as standard curve to determine FVIII R:Ag in 4 unknown plasma samples, supplied by the UK Reference Laboratory for Anticoagulant Reagent & Control; WHO Collaborating Center for Quality Assessment, in Blood Coagulation Testing for International Quality Control Survey in Blood Coagulation. The results were very satisfactory. The coefficient of variation was between 2.22-5.47 per cent when compared with other 29 laboratories around the world. These home made reference preparation are stable at least up to 30 months at -70 degrees C, and can be applied for calibration of unknown sample instead of the 1st British Standard for FVIII R:Ag, human 66/355.