Publication: A novel enhanced green fluorescent protein (EGFP)-K562 flow cytometric method for measuring natural killer (NK) cell cytotoxic activity
Issued Date
2003-01-15
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ISSN
00221759
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2-s2.0-0037438101
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Immunological Methods. Vol.272, No.1-2 (2003), 189-197
Suggested Citation
Wannee Kantakamalakul, Jiraporn Jaroenpool, Kovit Pattanapanyasat A novel enhanced green fluorescent protein (EGFP)-K562 flow cytometric method for measuring natural killer (NK) cell cytotoxic activity. Journal of Immunological Methods. Vol.272, No.1-2 (2003), 189-197. doi:10.1016/S0022-1759(02)00505-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20932
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Title
A novel enhanced green fluorescent protein (EGFP)-K562 flow cytometric method for measuring natural killer (NK) cell cytotoxic activity
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Abstract
Enhanced green fluorescent protein (EGFP) was stably expressed in human erythroleukaemia K562 cells (EGFP-K562) and used as target cells for measurement of natural killer (NK) cell cytotoxicity by flow cytometry. The compromised EGFP-K562 target cells were stained with propidium iodide (PI) and showed dual (green-red) fluorescent. Although the kinetic study demonstrated that the optimal incubation time for the assay was 4 h, a 2-h incubation period also gave comparable results. This new technique correlated strongly with the standard chromium (51Cr) release assay at the correlation coefficients of 0.87 and 0.89 at p-value <0.001 for 2- and 4-h incubation times, respectively. The EGFP-K562 stable cell line provides a novel method to measure NK cytotoxicity by flow cytometry without pre-staining or pre-labeling target cells. © 2002 Elsevier Science B.V. All rights reserved.