Publication: Analysis of the expression and antioxidant activity of 2-Cys peroxiredoxin protein in Fasciola gigantica
Issued Date
2014-01-01
Resource Type
ISSN
10902449
00144894
00144894
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2-s2.0-84896964272
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.140, No.1 (2014), 24-32
Suggested Citation
Kant Sangpairoj, Narin Changklungmoa, Rapeepun Vanichviriyakit, Prasert Sobhon, Kulathida Chaithirayanon Analysis of the expression and antioxidant activity of 2-Cys peroxiredoxin protein in Fasciola gigantica. Experimental Parasitology. Vol.140, No.1 (2014), 24-32. doi:10.1016/j.exppara.2014.02.017 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34004
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Title
Analysis of the expression and antioxidant activity of 2-Cys peroxiredoxin protein in Fasciola gigantica
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Abstract
2-Cys peroxiredoxin (Prx) is the main antioxidant enzyme in Fasciola species for detoxifying hydrogen peroxide which is generated from the hosts' immune effector cells and the parasites' own metabolism. In this study, the recombinant Prx protein from Fasciola gigantica (rFgPrx-2) was expressed and purified in a prokaryotic expression system. This recombinant protein with molecular weight of 26. kDa was enzymatically active in reduction of hydrogen peroxide both in presence of thioredoxin and glutathione systems, and also protected the supercoiled plasmid DNA from oxidative damage in metal-catalyzed oxidation (MCO) system in a concentration-dependent manner. By immunoblotting, using antibody against rFgPrx-2 as probe, a native FgPrxs, whose MW at 25. kDa, was detected in all developmental stages of the parasite. Concentrations of native FgPrxs were increasing in all stages reaching highest level in adult stage. The antibody also showed cross reactivities with corresponding proteins in some cattle helminthes. Natural antibody to FgPrxs could be detected in the sera of mice at 3 and 4. weeks after infection with F. gigantica metacercariae. By immunofluorescence, FgPrxs was highly expressed in tegument and tegumental cells, parenchyma, moderately expressed in cecal epithelial cells in early, juvenile and adult worms. Furthermore, FgPrxs was also detected in the female reproductive organs, including eggs, ovary, vitelline cells, and testis, suggesting that FgPrxs might play an essential role in protecting parasite's tissues from free radical attack during their life cycle. Thus, FgPrxs is one potential candidate for drug therapy and vaccine development. © 2014 Elsevier Inc.