Publication: Simultaneous analysis of metformin and cyanoguanidine by capillary zone electrophoresis and its application in a stability study
Issued Date
2014-01-01
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ISSN
16159314
16159306
16159306
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2-s2.0-84903716414
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Separation Science. Vol.37, No.13 (2014), 1687-1693
Suggested Citation
Athiporn Doomkaew, Brompoj Prutthiwanasan, Leena Suntornsuk Simultaneous analysis of metformin and cyanoguanidine by capillary zone electrophoresis and its application in a stability study. Journal of Separation Science. Vol.37, No.13 (2014), 1687-1693. doi:10.1002/jssc.201400310 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33578
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Title
Simultaneous analysis of metformin and cyanoguanidine by capillary zone electrophoresis and its application in a stability study
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Abstract
A capillary zone electrophoresis method was established for stability study of metformin (MET). MET and cyanoguanidine (CGN; a major degradation product) were well separated (with a resolution of 38.9) in 40 mM citrate buffer (pH 6.7) using a fused-silica capillary with an effective length of 60 cm and an inner diameter of 50 μm, injection at 50 mbar for 5 s at 30°C with an applied voltage of 15 kV and diode array detection at 214 nm. Method validation showed good linearity (r<sup>2</sup> > 0.99), precision (%RSDs < 1.98%), and accuracy (%recovery between 98.3 and 100.9%). Limits of detection and quantification were <30 and 100 μg/mL, respectively. The method was robust upon alteration of pH and voltage (%RSDs < 1.99%). Stability profiles of metformin from 11 stress conditions and the degradation kinetics could be established, using the simple capillary zone electrophoresis system. A mechanism for the degradation of MET was also proposed. MET was stable in neutral hydrolysis, but degraded under alkaline hydrolysis and oxidation. Under both conditions, CGN was quantified as the degradation product. An assay of MET in raw material and tablets showed that content of the drugs in all samples met the requirements of pharmacopeias and CGN was not detected. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.