Publication:
Multidrug-resistant Neisseria gonorrhoeae infection in heterosexual men with reduced susceptibility to ceftriaxone, first report in Thailand

Issued Date

2021-12-01

Resource Type

Article

ISSN

20452322

DOI

10.1038/s41598-021-00675-y

Other identifier(s)

2-s2.0-85118562735

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Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Scientific Reports. Vol.11, No.1 (2021)

Suggested Citation

Naris Kueakulpattana, Dhammika Leshan Wannigama, Sirirat Luk-in, Parichart Hongsing, Cameron Hurst, Vishnu Nayak Badavath, Piroon Jenjaroenpun, Thidathip Wongsurawat, Nipat Teeratakulpisan, Stephen J. Kerr, Shuichi Abe, Phatthranit Phattharapornjaroen, Aye Mya Sithu Shein, Thammakorn Saethang, Naphat Chantaravisoot, Mohan Amarasiri, Paul G. Higgins, Tanittha Chatsuwan Multidrug-resistant Neisseria gonorrhoeae infection in heterosexual men with reduced susceptibility to ceftriaxone, first report in Thailand. Scientific Reports. Vol.11, No.1 (2021). doi:10.1038/s41598-021-00675-y Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79203

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Title

Multidrug-resistant Neisseria gonorrhoeae infection in heterosexual men with reduced susceptibility to ceftriaxone, first report in Thailand

Author(s)

Naris Kueakulpattana
 Dhammika Leshan Wannigama
 Sirirat Luk-in
 Parichart Hongsing
 Cameron Hurst
 Vishnu Nayak Badavath
 Piroon Jenjaroenpun
 Thidathip Wongsurawat
 Nipat Teeratakulpisan
 Stephen J. Kerr
 Shuichi Abe
 Phatthranit Phattharapornjaroen
 Aye Mya Sithu Shein
 Thammakorn Saethang
 Naphat Chantaravisoot
 Mohan Amarasiri
 Paul G. Higgins
 Tanittha Chatsuwan

Other Contributor(s)

Yamagata Prefectural Central Hospital
 Medizinische Fakultät
 Siriraj Hospital
 Mae Fah Luang University Hospital
 UWA Medical School
 Chitkara University, Punjab
 Thai Red Cross Agency
 Chulalongkorn University
 QIMR Berghofer Medical Research Institute
 Kasetsart University
 Mae Fah Luang University
 The HIV Netherlands Australia Thailand Research Collaboration
 Faculty of Medicine Ramathibodi Hospital, Mahidol University
 Mahidol University
 Hebrew University of Jerusalem
 Kitasato University
 Partner Site Bonn-Cologne

Abstract

The global rapid emergence of azithromycin/ceftriaxone resistant Neisseria gonorrhoeae threatens current recommend azithromycin/ceftriaxone dual therapy for gonorrhea to ensure effective treatment. Here, we identified the first two N. gonorrhoeae isolates with decreased ceftriaxone susceptibility in Thailand. Among 134 N. gonorrhoeae isolates collected from Thai Red Cross Anonymous Clinic, Bangkok, two isolates (NG-083 and NG-091) from urethral swab in male heterosexual patients had reduced susceptibility to ceftriaxone (MICs of 0.125 mg/L). Both were multidrug resistant and strong biofilm producers with ceftriaxone tolerance (MBEC > 128 mg/L). NG-083 and NG-091 remained susceptible to azithromycin (MIC of 1 mg/L and 0.5 mg/L, respectively). Reduced susceptibility to ceftriaxone was associated with alterations in PBP2, PBP1, PorB, MtrR, and mtrR promoter region. NG-083 belonged to sequence type (ST) 7235 and NG-091 has new allele number of tbpB with new ST. Molecular docking revealed ceftriaxone weakly occupied the active site of mosaic XXXIV penicillin-binding protein 2 variant in both isolates. Molecular epidemiology results revealed that both isolates display similarities with isolates from UK, USA, and The Netherlands. These first two genetically related gonococcal isolates with decreased ceftriaxone susceptibility heralds the threat of treatment failure in Thailand, and importance of careful surveillance.

Keyword(s)

Multidisciplinary

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/79203

Collections

Scopus 2021