Publication: Identification and differentiation of human schistosomes by polymerase chain reaction
Issued Date
2010-03-01
Resource Type
ISSN
10902449
00144894
00144894
Other identifier(s)
2-s2.0-76049128113
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.124, No.3 (2010), 325-329
Suggested Citation
Naoko Kato-Hayashi, Masashi Kirinoki, Yukio Iwamura, Tamotsu Kanazawa, Viroj Kitikoon, Hajime Matsuda, Yuichi Chigusa Identification and differentiation of human schistosomes by polymerase chain reaction. Experimental Parasitology. Vol.124, No.3 (2010), 325-329. doi:10.1016/j.exppara.2009.11.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29255
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Title
Identification and differentiation of human schistosomes by polymerase chain reaction
Abstract
Recent increasing number of travelers, immigrants and foreign workers from schistosomiasis endemic area has thus resulted in the importation of schistosomiasis to non-endemic countries. To avoid ova-induced pathogenicity, sensitive and specific diagnostic means at an early stage of infection are therefore crucial. In this study, we developed polymerase chain reaction (PCR) primers specific for human schistosome species. The PCR products were obtained in a species-specific manner (479. bp, Schistosoma mansoni; 365. bp, S. haematobium; 614. bp, S. japonicum; 303. bp, S. mekongi) and were detectable from 0.01. pg of total worm DNA (S. haematobium, S. japonicum, S. mekongi). The primer sets were also available for multiplex use. Although some difficulties were experienced in amplifying the parasite DNA from the infected animals, schistosome DNA could be detected from one day post infection. The PCR method described herein will therefore be beneficial to detect human schistosomiasis, after some improvements in this method. © 2009 Elsevier Inc.