Publication:
Biochemical and ultrastructural characterizations of nucleoprotamine in human sperm heads treated with micrococcal nuclease and salt

Issued Date

1982-01-01

Resource Type

Article

ISSN

15543919
01487280

DOI

10.1002/mrd.1120060307

Other identifier(s)

2-s2.0-0020467133

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Gamete Research. Vol.6, No.3 (1982), 235-255

Suggested Citation

Nongnuj Tanphaichitr, Prasert Sobhon, Panpaka Chalermisarachai, Chutima Chutatape Biochemical and ultrastructural characterizations of nucleoprotamine in human sperm heads treated with micrococcal nuclease and salt. Gamete Research. Vol.6, No.3 (1982), 235-255. doi:10.1002/mrd.1120060307 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30297

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Title

Biochemical and ultrastructural characterizations of nucleoprotamine in human sperm heads treated with micrococcal nuclease and salt

Author(s)

Nongnuj Tanphaichitr
 Prasert Sobhon
 Panpaka Chalermisarachai
 Chutima Chutatape

Other Contributor(s)

Mahidol University

Abstract

Preparation of human sperm heads containing mainly nucleoprotamines as their chromatin constituent was described. Acrosome‐depleted human sperm heads were treated with micrococcal nuclease followed with 2 M NaCl. The treatment specifically removed nucleohistone as small chromatin fragments from the human sperm heads. The nuclease‐NaCl‐treated sperm head pellets primarily contained nuclease‐resistant nucleoprotamine as their chromatin. Transmission electron microscopic study showed that there were two populations of the nuclease‐NaCl‐treated human sperm head pellets. One population had thick nucleoprotamine cords of ˜650–1,200 Å in diameter, and the other had thin nucleoprotamine cords of ˜330–420 Å. Copyright © 1982 Alan R. Liss, Inc.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/30297

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Scopus 1969-1990