Publication:
Compensatory increase in ahpC gene expression and its role in protecting Burkholderia pseudomallei against reactive nitrogen intermediates

1

Issued Date

2003-12-01

Resource Type

Article

ISSN

03028933

DOI

10.1007/s00203-003-0621-9

Other identifier(s)

2-s2.0-0346364903

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Archives of Microbiology. Vol.180, No.6 (2003), 498-502

Suggested Citation

Suvit Loprasert, Ratiboot Sallabhan, Wirongrong Whangsuk, Skorn Mongkolsuk Compensatory increase in ahpC gene expression and its role in protecting Burkholderia pseudomallei against reactive nitrogen intermediates. Archives of Microbiology. Vol.180, No.6 (2003), 498-502. doi:10.1007/s00203-003-0621-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/20861

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Title

Compensatory increase in ahpC gene expression and its role in protecting Burkholderia pseudomallei against reactive nitrogen intermediates

Author(s)

Suvit Loprasert
 Ratiboot Sallabhan
 Wirongrong Whangsuk
 Skorn Mongkolsuk

Other Contributor(s)

Chulabhorn Research Institute
 Mahidol University

Abstract

In the human pathogen Burkholderia pseudomallei, katG encodes the antioxidant defense enzyme catalase-peroxidase. Interestingly, a B. pseudomallei mutant, disrupted in katG, is hyperresistant to organic hydroperoxide. This hyperresistance is due to the compensatory expression of the alkyl hydroperoxide reductase gene (ahpC) and depends on a global regulator OxyR. The KatG-deficient mutant is also highly resistant to reactive nitrogen intermediates (RNI). When overproduced, the B. pseudomallei AhpC protein, protected cells against killing by RNI. The levels of resistance to both organic peroxide and RNI returned to those of the wild-type when the katG mutant was complemented with katG. These studies establish the partially overlapping defensive activities of KatG and AhpC.

Keyword(s)

Immunology and Microbiology

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URI

https://repository.li.mahidol.ac.th/handle/123456789/20861

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Scopus 2001-2005