Publication: Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
Issued Date
2021-12-01
Resource Type
ISSN
20452322
Other identifier(s)
2-s2.0-85107812506
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Scientific Reports. Vol.11, No.1 (2021)
Suggested Citation
Atsushi Yamanaka, Hisham Ahmed Imad, Weerapong Phumratanaprapin, Juthamas Phadungsombat, Eiji Konishi, Tatsuo Shioda Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients. Scientific Reports. Vol.11, No.1 (2021). doi:10.1038/s41598-021-91793-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/79238
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
Abstract
Dengue virus (DENV) causes dengue fever (DF) and dengue hemorrhagic fever in humans. Some DF patients suddenly develop severe symptoms around the defervescent period. Although the pathogenic mechanism of the severe symptoms has not been fully elucidated, the viremia level in the early phase has been shown to correlate with the disease severity. One of the hypotheses is that a phenomenon called antibody-dependent enhancement (ADE) of infection leads to high level of viremia. To examine the plausibility of this hypothesis, we examined the relationship between in vitro ADE activity and in vivo viral load quantity in six patients with dengue diseases. Blood samples were collected at multiple time points between the acute and defervescent phases, and the balance between neutralizing and enhancing activities against the autologous and prototype viruses was examined. As the antibody levels against DENV were rapidly increased, ADE activity was decreased over time or partially maintained against some viruses at low serum dilution. In addition, positive correlations were observed between ADE activity representing in vitro progeny virus production and viremia levels in patient plasma samples. The measurement of ADE activity in dengue-seropositive samples may help to predict the level of viral load in the subsequent DENV infection.