Publication: IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria
Accepted Date
2009-12-10
Issued Date
2009-12-10
Copyright Date
2009
Resource Type
Language
eng
ISSN
1475-2875 (electronic)
Rights
Mahidol University
Rights Holder(s)
BioMed Central
Bibliographic Citation
Tangteerawatana P, Perlmann H, Hayano M, Kalambaheti T, Troye-Blomberg M, Khusmith S. IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria. Malar J. 2009 Dec 10;8:286.
Suggested Citation
Piyatida Tangteerawatana, ปิยะธิดา ตั้งธีระวัฒนะ, Perlmann, Hedvig, Hayano, Masashi, Thareerat Kalambaheti, ธารีรัตน์ กะลัมพะเหติ, Troye-Blomberg, Marita, Srisin Khusmith, ศรีสิน คูสมิทธิ์ IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria. Tangteerawatana P, Perlmann H, Hayano M, Kalambaheti T, Troye-Blomberg M, Khusmith S. IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria. Malar J. 2009 Dec 10;8:286.. doi:10.1186/1475-2875-8-286 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/696
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Title
IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria
Corresponding Author(s)
Abstract
BACKGROUND: The IL4-590 gene polymorphism has been shown to be associated with
elevated levels of anti-Plasmodium falciparum IgG antibodies and parasite
intensity in the malaria protected Fulani of West Africa. This study aimed to
investigate the possible impact of IL4-590C/T polymorphism on anti-P. falciparum
IgG subclasses and IgE antibodies levels and the alteration of malaria severity
in complicated and uncomplicated malaria patients with or without previous
malaria experiences.
METHODS: Anti-P.falciparum IgG subclasses and IgE antibodies in plasma of
complicated and uncomplicated malaria patients with or without previous malaria
experiences were analysed using ELISA. IL4-590 polymorphisms were genotyped using
RFLP-PCR. Statistical analyses of the IgG subclass levels were done by Oneway
ANOVA. Genotype differences were tested by Chi-squared test.
RESULTS: The IL4-590T allele was significantly associated with anti-P. falciparum
IgG3 antibody levels in patients with complicated (P = 0.031), but not with
uncomplicated malaria (P = 0.622). Complicated malaria patients with previous
malaria experiences carrying IL4-590TT genotype had significantly lower levels of
anti-P. falciparum IgG3 (P = 0.0156), while uncomplicated malaria patients with
previous malaria experiences carrying the same genotype had significantly higher
levels (P = 0.0206) compared to their IL4-590 counterparts. The different anti-P.
falciparum IgG1 and IgG3 levels among IL4 genotypes were observed. Complicated
malaria patients with previous malaria experiences tended to have lower IgG3
levels in individuals carrying TT when compared to CT genotypes (P = 0.075). In
contrast, complicated malaria patients without previous malaria experiences
carrying CC genotype had significantly higher anti-P. falciparum IgG1 than those
carrying either CT or TT genotypes (P = 0.004, P = 0.002, respectively).
CONCLUSION: The results suggest that IL4-590C or T alleles participated
differently in the regulation of anti-malarial antibody isotype profiles in
primary and secondary malaria infection and, therefore, could play an important
role in alteration of malaria severity.