Publication: Characterization of Argonaute cDNA from Penaeus monodon and implication of its role in RNA interference
Issued Date
2008-03-21
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-38649089779
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.367, No.4 (2008), 768-774
Suggested Citation
Manasave Dechklar, Apinunt Udomkit, Sakol Panyim Characterization of Argonaute cDNA from Penaeus monodon and implication of its role in RNA interference. Biochemical and Biophysical Research Communications. Vol.367, No.4 (2008), 768-774. doi:10.1016/j.bbrc.2008.01.031 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18947
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Characterization of Argonaute cDNA from Penaeus monodon and implication of its role in RNA interference
Author(s)
Other Contributor(s)
Abstract
RNA interference (RNAi) has recently become a promising strategy for therapeutic of several viral diseases including those in the black tiger shrimp Penaeus monodon. However, the protein components that play role in RNAi in P. monodon have not yet been identified. Here, we report the cloning and functional characterization of a cDNA encoding Argonaute, a principal constituent of RNAi pathway in P. monodon. P. monodon's Argonaute (Pem-AGO) exhibited the two signature domains, PAZ and PIWI. Substantial level of Pem-ago expression could be suppressed by double-stranded RNA (dsRNA) that targeted PAZ coding sequence in shrimp primary culture of Oka cells. The Pem-ago depleted cells showed impaired RNAi as the expression of an endogenous gene was rescued from the dsRNA-mediated silencing in these cells. Our results imply that Pem-ago is required for effective RNAi in P. monodon and thus identify the first protein constituent of RNAi machinery in penaeid shrimp. © 2008 Elsevier Inc. All rights reserved.