Publication: In vitro vessel-forming capacity of endothelial progenitor cells in high glucose conditions
Issued Date
2012-03-01
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ISSN
14320584
09395555
09395555
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2-s2.0-84857373475
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Mahidol University
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SCOPUS
Bibliographic Citation
Annals of Hematology. Vol.91, No.3 (2012), 311-320
Suggested Citation
Chaiwat Jiraritthamrong, Pakpoom Kheolamai, Yaowalak U-Pratya, Methichit Chayosumrit, Aungkura Supokawej, Sirikul Manochantr, Chairat Tantrawatpan, Hathaitip Sritanaudomchai, Surapol Issaragrisil In vitro vessel-forming capacity of endothelial progenitor cells in high glucose conditions. Annals of Hematology. Vol.91, No.3 (2012), 311-320. doi:10.1007/s00277-011-1300-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14924
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Title
In vitro vessel-forming capacity of endothelial progenitor cells in high glucose conditions
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Abstract
In type 2 diabetes, the impairment of vascular repair processes and angiogenesis are due to endothelial progenitor cell (EPC) dysfunction. In this study, we established a quantitative methodology to assess EPC function by using an in vitro 5-(6)-carboxyfluorescein diacetate succinimidyl ester-labeling vessel formation assay. The EPCs were cultured in three different glucose concentrations (100, 189.5, and 295.5 mg/dl of d-glucose) representing normal control and diabetes with either good or poor glycemic control, respectively. We found that the in vitro vessel-forming capacity was impaired in EPCs cultured in high glucose concentrations compared to normal control (43.4 ± 0.8% and 34.7 ± 0.7% vs. 50.8 ± 2.1%). We further studied expression of various genes involved in vessel formation. There was a lower level of angiopoietin 1 gene expression in EPCs cultured in high glucose concentrations. The addition of recombinant angiopoietin 1 significantly increased the vessel-forming capacity of EPCs cultured in high glucose concentration (35.3 ± 2.0% to 48.8 ± 2.7%), whereas the addition of angiopoietin 2 (a competitive inhibitor of angiopoietin 1) impaired the vessel-forming capacity of EPCs cultured in normal glucose concentration (51.8 ± 1.3% to 41.3 ± 0.6%). We conclude that the in vitro vessel-forming capacity of EPCs cultured in high glucose concentration is impaired due to low levels of angiopoietin 1. © 2011 Springer-Verlag.