Publication:
African green monkeys recapitulate the clinical experience with replication of live attenuated pandemic influenza virus vaccine candidates

dc.contributor.authorYumiko Matsuokaen_US
dc.contributor.authorAmorsolo Suguitanen_US
dc.contributor.authorMarlene Orandleen_US
dc.contributor.authorMyeisha Paskelen_US
dc.contributor.authorKobporn Boonnaken_US
dc.contributor.authorDonald J. Gardneren_US
dc.contributor.authorFriederike Feldmannen_US
dc.contributor.authorHeinz Feldmannen_US
dc.contributor.authorMichael Marinoen_US
dc.contributor.authorHong Jinen_US
dc.contributor.authorGeorge Kembleen_US
dc.contributor.authorKanta Subbaraoen_US
dc.contributor.otherNational Institute of Allergy and Infectious Diseasesen_US
dc.contributor.otherNational Institutes of Health, Bethesdaen_US
dc.contributor.otherMedImmune, Inc.en_US
dc.contributor.otherAmerican University of the Caribbeanen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherFDA Center for Biologics Evaluation and Researchen_US
dc.contributor.other3-V Biosciences, Inc.en_US
dc.date.accessioned2018-11-09T02:26:40Z
dc.date.available2018-11-09T02:26:40Z
dc.date.issued2014-01-01en_US
dc.description.abstractLive attenuated cold-adapted (ca) H5N1, H7N3, H6N1, and H9N2 influenza vaccine viruses replicated in the respiratory tract of mice and ferrets, and 2 doses of vaccines were immunogenic and protected these animals from challenge infection with homologous and heterologous wild-type (wt) viruses of the corresponding subtypes. However, when these vaccine candidates were evaluated in phase I clinical trials, there were inconsistencies between the observations in animal models and in humans. The vaccine viruses did not replicate well and immune responses were variable in humans, even though the study subjects were seronegative with respect to the vaccine viruses before vaccination. Therefore, we sought a model that would better reflect the findings in humans and evaluated African green monkeys (AGMs) as a nonhuman primate model. The distribution of sialic acid (SA) receptors in the respiratory tract of AGMs was similar to that in humans. We evaluated the replication of wt and ca viruses of avian influenza (AI) virus subtypes H5N1, H6N1, H7N3, and H9N2 in the respiratory tract of AGMs. All of the wt viruses replicated efficiently, while replication of the ca vaccine viruses was restricted to the upper respiratory tract. Interestingly, the patterns and sites of virus replication differed among the different subtypes. We also evaluated the immunogenicity and protective efficacy of H5N1, H6N1, H7N3, and H9N2 ca vaccines. Protection from wt virus challenge correlated well with the level of serum neutralizing antibodies. Immune responses were slightly better when vaccine was delivered by both intranasal and intratracheal delivery than when it was delivered intranasally by sprayer. We conclude that live attenuated pandemic influenza virus vaccines replicate similarly in AGMs and human subjects and that AGMs may be a useful model to evaluate the replication of ca vaccine candidates. © 2014, American Society for Microbiology.en_US
dc.identifier.citationJournal of Virology. Vol.88, No.14 (2014), 8139-8152en_US
dc.identifier.doi10.1128/JVI.00425-14en_US
dc.identifier.issn10985514en_US
dc.identifier.issn0022538Xen_US
dc.identifier.other2-s2.0-84904658491en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/34084
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84904658491&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.titleAfrican green monkeys recapitulate the clinical experience with replication of live attenuated pandemic influenza virus vaccine candidatesen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84904658491&origin=inwarden_US

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