Publication:
Variation of expression defects in cell surface 190-kDa protein antigen of Streptococcus mutans

dc.contributor.authorJinthana Lapirattanakulen_US
dc.contributor.authorRyota Nomuraen_US
dc.contributor.authorMichiyo Matsumoto-Nakanoen_US
dc.contributor.authorRatchapin Srisatjaluken_US
dc.contributor.authorTakashi Ooshimaen_US
dc.contributor.authorKazuhiko Nakanoen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherOsaka Universityen_US
dc.contributor.otherOkayama Universityen_US
dc.date.accessioned2018-11-23T10:17:30Z
dc.date.available2018-11-23T10:17:30Z
dc.date.issued2015-05-01en_US
dc.description.abstract© 2015 Elsevier GmbH. Streptococcus mutans, which consists of four serotypes, c, e, f, and k, possesses a 190-kDa cell surface protein antigen (PA) for initial tooth adhesion. We used Western blot analysis to determine PA expression in 750 S. mutans isolates from 150 subjects and found a significantly higher prevalence of the isolates with PA expression defects in serotypes f and k compared to serotypes c and e. Moreover, the defect patterns could be classified into three types; no PA expression on whole bacterial cells and in their supernatant samples (Type N1), PA expression mainly seen in supernatant samples (Type N2), and only low expression of PA in the samples of whole bacterial cells (Type W). The underlying reasons for the defects were mutations in the gene encoding PA as well as in the transcriptional processing of this gene for Type N1, defects in the sortase gene for Type N2, and low mRNA expression of PA for Type W. Since cellular hydrophobicity and phagocytosis susceptibility of the PA-defective isolates were significantly lower than those of the normal expression isolates, the potential implication of such defective isolates in systemic diseases involving bacteremia other than dental caries was suggested. Additionally, multilocus sequence typing was utilized to characterize S. mutans clones that represented a proportion of isolates with PA defects of 65-100%. Therefore, we described the molecular basis for variation defects in PA expression of S. mutans. Furthermore, we also emphasized the strong association between PA expression defects and serotypes f and k as well as the clonal relationships among these isolates.en_US
dc.identifier.citationInternational Journal of Medical Microbiology. Vol.305, No.3 (2015), 383-391en_US
dc.identifier.doi10.1016/j.ijmm.2015.03.001en_US
dc.identifier.issn16180607en_US
dc.identifier.issn14384221en_US
dc.identifier.other2-s2.0-84955710378en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/36111
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84955710378&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleVariation of expression defects in cell surface 190-kDa protein antigen of Streptococcus mutansen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84955710378&origin=inwarden_US

Files

Collections