Publication: QCM-Based DNA biosensor for Salmonella typhimurium detection
Issued Date
2012-12-01
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2-s2.0-84875718064
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Mahidol University
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SCOPUS
Bibliographic Citation
2012 IEEE International Conference on Electron Devices and Solid State Circuit, EDSSC 2012. (2012)
Suggested Citation
Uraiwan Waiwijit, Kata Jaruwongrungsee, Nipa Chokesajjawatee, Jurairat Promjai, Tanom Lomas, Pornpimol Sritongkham, Adisorn Tuantranont QCM-Based DNA biosensor for Salmonella typhimurium detection. 2012 IEEE International Conference on Electron Devices and Solid State Circuit, EDSSC 2012. (2012). doi:10.1109/EDSSC.2012.6482844 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/14022
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Title
QCM-Based DNA biosensor for Salmonella typhimurium detection
Abstract
This work demonstrated a QCM biosensor for the detection of Salmonella typhimurium using the specific DNA probe. A thiol DNA probe was immobilized onto quartz surface coated with gold through self-assembly via Au-S bonding formation. 6-mercapto-1-hexanol (MCH) was used as blocking reagent against non-specific DNA binding. Target DNA with 94 bp (single strand) was hybridized with its probe resulted in a decrease in the resonance frequency of the QCM biosensor. It was demonstrated that the QCM biosensor could recognize the target DNA sequence within 40-70 min. The frequency shift signal was distinguishable along with the increase of target concentration from 0.1 to 1.0 μM. © 2012 IEEE.