Publication: Hepatitis C virus nonstructural 3 protein: Recombinant NS3 protein of the Thai isolates as an antigen in a diagnostic assay
Issued Date
2002-09-01
Resource Type
ISSN
0125877X
Other identifier(s)
2-s2.0-0036771399
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.20, No.3 (2002), 161-166
Suggested Citation
Duangjit Kanistanon, Patimaporn Wongprompitak, Tararaj Dharakul, Sirirurg Songsivilai Hepatitis C virus nonstructural 3 protein: Recombinant NS3 protein of the Thai isolates as an antigen in a diagnostic assay. Asian Pacific Journal of Allergy and Immunology. Vol.20, No.3 (2002), 161-166. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20361
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Hepatitis C virus nonstructural 3 protein: Recombinant NS3 protein of the Thai isolates as an antigen in a diagnostic assay
Other Contributor(s)
Abstract
Nonstructural 3 (NS3) protein of hepatitis C virus (HCV) is one of the antigens commonly used in diagnostic assays for antibody to hepatitis C virus. However, immune response to the NS3 protein from one genotype may not cross-react with that from other genotypes. In the development of an anti-HCV assay, the NS3 genes from genotypes 1 and 3 commonly found in Thailand were amplified and cloned into a bacterial expression system. These recombinant NS3 proteins were immunogenic and reacted with plasma samples of Thai patients infected with various HCV genotypes. Interestingly, the NS3 proteins from the Thai genotypes could react with 3 plasma samples from HCV infected Thai blood donors, which could not bind to the NS3.1 protein in the commercial HCV immunoblot kit using antigen from HCV genotype 1. This finding supports our prior observation that the appropriate HCV antigens used in a diagnostic assay should be derived from the virus genotypes commonly found in that geographical region.