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Short report: Comparison of performance of serum and plasma in panbio dengue and Japanese encephalitis virus enzyme-linked immunosorbent assays

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dc.contributor.authorStuart D. Blacksellen_US
dc.contributor.authorSue J. Leeen_US
dc.contributor.authorAnisone Chanthongthipen_US
dc.contributor.authorThaksinaporn Taojaikongen_US
dc.contributor.authorSoulignasack Thongpaseuthen_US
dc.contributor.authorTanja Hübscheren_US
dc.contributor.authorPaul N. Newtonen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherUniversity of Oxforden_US
dc.contributor.otherMahosot Hospitalen_US
dc.contributor.otherHohmad Clinicen_US
dc.date.accessioned2018-06-11T04:52:02Z
dc.date.available2018-06-11T04:52:02Z
dc.date.issued2012-09-01en_US
dc.description.abstractWe examined the comparative performance of serum and plasma (in dipotassium EDTA) in Panbio Dengue enzyme-linked immunosorbent assays (ELISAs) for detection of non-structural protein 1 (NS1), IgM, and IgG, and a dengue/Japanese encephalitis virus (JEV) combination IgM ELISA in a prospective series of 201 patients with suspected dengue in Laos. Paired comparisons of medians from serum and plasma samples were not significantly different for Dengue IgM, and NS1 which had the highest number of discordant pairs (both 2%; P = 0.13 and P = 0.25, respectively). Comparison of qualitative final diagnostic interpretations for serum and plasma samples were not significantly different: only 1.5% (3 of 201 for Dengue/JEV IgM and Dengue IgG) and 2.0% (4 of 201; IgM and NS1) showed discordant pairs. These results demonstrate that plasma containing EDTA is suitable for use in these ELISAs. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.en_US
dc.identifier.citationAmerican Journal of Tropical Medicine and Hygiene. Vol.87, No.3 (2012), 573-575en_US
dc.identifier.doi10.4269/ajtmh.2012.12-0043en_US
dc.identifier.issn00029637en_US
dc.identifier.other2-s2.0-84865976395en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/14276
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865976395&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleShort report: Comparison of performance of serum and plasma in panbio dengue and Japanese encephalitis virus enzyme-linked immunosorbent assaysen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84865976395&origin=inwarden_US

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