Publication: Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation
Issued Date
2006-05-01
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ISSN
01660934
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2-s2.0-33645284994
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.133, No.2 (2006), 180-184
Suggested Citation
Parin Chaivisuthangkura, Phiromsak Phattanapaijitkul, Nitaya Thammapalerd, Sombat Rukpratanporn, Siwaporn Longyant, Weerawan Sithigorngul, Paisarn Sithigorngul Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation. Journal of Virological Methods. Vol.133, No.2 (2006), 180-184. doi:10.1016/j.jviromet.2005.11.012 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23336
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Title
Development of a polyclonal antibody specific to VP19 envelope protein of white spot syndrome virus (WSSV) using a recombinant protein preparation
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Abstract
The VP19 gene encoding a structural envelope protein of white spot syndrome virus was cloned into an expression vector and introduced into E. coli. The objective was to produce a recombinant VP19 structural protein. After induction, the recombinant VP19 protein (rVP19) was produced, purified by SDS-PAGE and used for immunization of Swiss mice for polyclonal antibody production. The mouse anti rVP19 antiserum had specific immunoreactivity to the viral antigen in WSSV infected Penaeus monodon as verified by immunohistochemistry and Western blot. The production of monoclonal antibodies against this rVP19 may be useful in order to combine with anti-VP28 monoclonal antibodies for enhancing the sensitivity of various WSSV serological assays. © 2006 Elsevier B.V. All rights reserved.