Publication: Analysis of non-photochemical energy dissipating processes in wild type dunaliella salina (green algae) and in zea1, a mutant constitutively accumulating zeaxanthin
Issued Date
2009-07-01
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09189440
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2-s2.0-67650594949
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Plant Research. Vol.122, No.4 (2009), 465-476
Suggested Citation
Rutanachai Thaipratum, Anastasios Melis, Jisnuson Svasti, Kittisak Yokthongwattana Analysis of non-photochemical energy dissipating processes in wild type dunaliella salina (green algae) and in zea1, a mutant constitutively accumulating zeaxanthin. Journal of Plant Research. Vol.122, No.4 (2009), 465-476. doi:10.1007/s10265-009-0229-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27005
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Title
Analysis of non-photochemical energy dissipating processes in wild type dunaliella salina (green algae) and in zea1, a mutant constitutively accumulating zeaxanthin
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Abstract
Generally there is a correlation between the amount of zeaxanthin accumulated within the chloroplast of oxygenic photosynthetic organisms and the degree of non-photochemical quenching (NPQ). Although constitutive accumulation of zeaxanthin can help protect plants from photo-oxidative stress, organisms with such a phenotype have been reported to have altered rates of NPQ induction. In this study, basic fluorescence principles and the routinely used NPQ analysis technique were employed to investigate excitation energy quenching in the unicellular green alga Dunaliella salina, in both wild type (WT) and a mutant, zea1, constitutively accumulating zeaxanthin under all growth conditions. The results showed that, in D. salina, NPQ is a multi-component process consisting of energy- or ΔpH-dependent quenching (qE), state-transition quenching (qT), and photoinhibition quenching (qI). Despite the vast difference in the amount of zeaxanthin in WT and the zea1 mutant grown under low light, the overall kinetics of NPQ induction were almost the same. Only a slight difference in the relative contribution of each quenching component could be detected. Of all the NPQ subcomponents, qE seemed to be the primary NPQ operating in this alga in response to short-term exposure to excessive irradiance. Whenever qE could not operate, i.e., in the presence of nigericin, or under conditions where the level of photon flux is beyond its quenching power, qT and/or qI could adequately compensate its photoprotective function. © 2009 The Botanical Society of Japan and Springer.