Publication: Immunodiagnosis of human trichinellosis using excretory-secretory (ES) antigen
Issued Date
1992-01-01
Resource Type
ISSN
14752697
0022149X
0022149X
Other identifier(s)
2-s2.0-0027102299
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Helminthology. Vol.66, No.4 (1992), 297-304
Suggested Citation
Wanpen Chaicumpa, Prasert Setasuban Immunodiagnosis of human trichinellosis using excretory-secretory (ES) antigen. Journal of Helminthology. Vol.66, No.4 (1992), 297-304. doi:10.1017/S0022149X00014759 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22245
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Title
Immunodiagnosis of human trichinellosis using excretory-secretory (ES) antigen
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Abstract
Infective first stage larvae of Trichinella spiralis were recovered from muscles of laboratory infected mice by digesting the muscles with 1% HC1-1% pepsin and collecting the larvae by modified Baerman's method. The larvae were cultivated in a serum-free medium for 18 h. The ES antigen obtained from the culture medium was used in an enzyme-linked immunosorbent assay (ELISA) for detecting IgG antibodies to T. spiralis in serum samples collected from three groups of individuals. The individuals of the first group were parasitologically confirmed trichinellosis patients, while those of group 2 were patients with other helminthiasis and group 3 were healthy, parasite-free individuals. The specificity of the assay was 100%. The sensitivity of the test was also 100% when performed on sera of group 1 collected at days 57 and 120 after infection. Sera collected earlier (day 23) and those collected 700 days after infection had negligible reactivity. Thus IgG-ELISA using ES antigen of the L1was useful not only for diagnosis but also in evaluation of cure. Western blot analysis revealed that specific antigens of T. spiralis were 94, 67, 63, and 39 kilodalton components. © 1992, Cambridge University Press. All rights reserved.