Publication: Ubiquitin-Conjugating Enzyme E2 L3 is Downregulated by the Chikungunya Virus nsP2 Protease
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Issued Date
2018-07-01
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ISSN
18628354
18628346
18628346
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2-s2.0-85034071599
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Mahidol University
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SCOPUS
Bibliographic Citation
Proteomics - Clinical Applications. Vol.12, No.4 (2018)
Suggested Citation
Suwipa Ramphan, Sarawut Khongwichit, Chonticha Saisawang, Duangnapa Kovanich, Albert J. Ketterman, Sukathida Ubol, Prasert Auewarakul, Sittiruk Roytrakul, Duncan R. Smith, Atichat Kuadkitkan Ubiquitin-Conjugating Enzyme E2 L3 is Downregulated by the Chikungunya Virus nsP2 Protease. Proteomics - Clinical Applications. Vol.12, No.4 (2018). doi:10.1002/prca.201700020 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45118
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Title
Ubiquitin-Conjugating Enzyme E2 L3 is Downregulated by the Chikungunya Virus nsP2 Protease
Abstract
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Purpose: Chikungunya virus (CHIKV) is a mosquito transmitted alphavirus that causes chikungunya fever in humans. The CHIKV non-structural protein 2 (nsP2) is a multifunctional protein that additionally modulates the host cell to dampen the innate immune response and inhibit other cellular processes. Experimental design: To further investigate the interactions of nsP2 with host cells, the protease domain of CHIKV nsP2 (nsP2-pro) is transfected into Hela cells, and differential protein expression is detected by 2D polyacrylamide gel electrophoresis. Results: A total of 21 differentially regulated (six upregulated, 15 downregulated) spots are observed, of which five are identified by mass spectrometry. The downregulation of one of the identified proteins, ubiquitin-conjugating enzyme E2 L3 (UBE2L3) is confirmed by western blotting of both nsP2-pro transfection and CHIKV natural infection, and the downregulation of UBE2L3 is additionally shown to require an enzymatically active nsP2 protease domain. Transfection of full length UBE2L3 into HEK293T/17 cells prior to CHIKV infection reduce levels of infection and E protein expression but do not alter RNA genome levels. Conclusion: These results suggest that UBE2L3 is a cellular target of the CHIKV nsP2 protease, and this possibly mediates the pathogenesis of chikungunya fever.