Publication: No abnormalities of reg1α and reg1β gene associated with diabetes mellitus
Issued Date
2002-01-19
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ISSN
01688227
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2-s2.0-0036138926
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Mahidol University
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SCOPUS
Bibliographic Citation
Diabetes Research and Clinical Practice. Vol.55, No.2 (2002), 105-111
Suggested Citation
Napatawn Banchuin, Watip Boonyasrisawat, Pinya Pulsawat, Sathit Vannasaeng, Chaicharn Deerochanawong, Sutin Sriussadaporn, Sirirat Ploybutr, Thawatchai Pasurakul, Pa thai Yenchitsomanus No abnormalities of reg1α and reg1β gene associated with diabetes mellitus. Diabetes Research and Clinical Practice. Vol.55, No.2 (2002), 105-111. doi:10.1016/S0168-8227(01)00321-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20085
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Title
No abnormalities of reg1α and reg1β gene associated with diabetes mellitus
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Abstract
In order to investigate whether there would be any association between abnormalities of either reg1α or reg1β gene and diabetes mellitus in man, these two genes were analyzed in 42 patients with type 1 diabetes mellitus, 12 with fibrocalculous pancreatopathy, 37 with type 2 diabetes mellitus, and 22 normal controls, by PCR-SSCP analysis and nucleotide sequencing technique. Polymorphism in the reg1α gene resulted in three mobility patterns in the PCR-SSCP analysis, due to nucleotide constituents at position -10 before exon 1 being either C/C, T/C or T/T. These three mobility patterns were observed in every group of subjects. The analysis of reg1β gene showed nucleotide substitutions in exon 4 in one patient, exon 5 in another patient with type 1 diabetes, and in exon 4 and intron 5 in one patient with fibrocalculous pancreatopathy. The nucleotide substitutions in exon 4 in the patient with type 1 diabetes and that with fibrocalculous pancreatopathy occurred at codons 103 and 84 while that in exon 5 in the patient with type 1 diabetes occurred at codon 141, changing the codons from CAT to CAC, GTG to GCG, and ACT to AAT and resulting in H103H silent, V84A and T141N missense mutations, respectively. In conclusion, using PCR-SSCP and nucleotide sequence analyses, we did not find any association between abnormalities of either reg1α or reg1β gene with any type of diabetes studied. Copyright © 2002 Elsevier Science Ireland Ltd.