This study evaluated the cytotoxicity of four single component dentin bonding agents: Syntac Single Component, Prime & Bond 2.1, Single Bond and One Up Bond F. The test materials were applied on dentin discs of dentin barrier models in the same way as in the clinical procedures recommended by each manufacturer. Cell viability of L 929 after exposure with the bonding agents was determined by MTT assay. The results revealed that cell survival of the first three bonding agents was 60%, while the fourth was an impressive 93%. This study showed that a total-etching bonding system is more cytotoxic than a self-etching bonding system.
