Publication: Host induced modification of dengue 2 virus surface antigens in Aedes albopictus cells
Issued Date
1973-12-01
Resource Type
ISSN
00383619
Other identifier(s)
2-s2.0-0015721411
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.4, No.4 (1973), 571-578
Suggested Citation
N. P. Salazar, S. Thomson Host induced modification of dengue 2 virus surface antigens in Aedes albopictus cells. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.4, No.4 (1973), 571-578. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/10158
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Title
Host induced modification of dengue 2 virus surface antigens in Aedes albopictus cells
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Abstract
Infection of Singh's Aedes albopictus (Aal) cell line with dengue virus type 2 (strain New Guinea C) was found to be CPE-negative and to result in the recovery of exclusively mature virus particles or rapidly sedimenting hemagglutinin (RHA) in sucrose gradient analysis. This DEN-2 (Aal)/RHA was compared with the products of mammalian cell infection [DEN-2 (MK2)/RHA] and Aal carrier culture (CHA) by polyacrylamide gel electrophoresis. The polypeptides of DEN-2 (Aal) and CHA were hereby characterized for the first time. The polypeptide components of variously derived RHA undergo qualitative and/or quantitative changes in electrophoretic behavior accordingly, i.e., DEN-2 (MK2) < DEN-2 (Aal) < CHA. The foregoing changes in virion surface antigens, as such, representing host derived modification, substantiate previously observed alterations in biological and immunological properties of DEN-2 in persistently infected mosquito cells. The implications of these findings on the possible behavior of virus in arthropodan and vertebrate host can not be overemphasized.
