Publication: Temperature Dependence of Plasmodium falciparum Erythrocytic Stage Development
Issued Date
2019-01-01
Resource Type
ISSN
00029637
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2-s2.0-85065509822
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.100, No.5 (2019), 1191-1195
Suggested Citation
Yutatirat Singhaboot, Srisuda Keayarsa, Nattaporn Piaraksa, Weerapong Phumratanaprapin, Parinya Kunawut, Arjen Dondorp, Kesinee Chotivanich Temperature Dependence of Plasmodium falciparum Erythrocytic Stage Development. American Journal of Tropical Medicine and Hygiene. Vol.100, No.5 (2019), 1191-1195. doi:10.4269/ajtmh.18-0894 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/51128
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Title
Temperature Dependence of Plasmodium falciparum Erythrocytic Stage Development
Abstract
Copyright © 2019 by The American Society of Tropical Medicine and Hygiene Plasmodium falciparum infection causes febrile illness and severe disease with multiple organ failure and death when treatment is delayed. Antipyretic treatment is standard, and inducing hypothermia has been proposed to protect the brain in cerebral malaria. Here, we investigated the temperature dependence of asexual-stage parasite development and parasite multiplication in vitro. Plasmodium falciparum laboratory strain TM267 was incubated for 2 hours (short exposure) or 48 hours (continuous exposure) at different temperatures (32°C, 34°C, 35°C, 38°C, 39°C, and 40°C). The starting parasite developmental stage (ring, trophozoite, or schizont) varied between experiments. The parasite multiplication rate (PMR) was reduced under both hyper- and hypothermic conditions; after continuous exposure, the mean PMR ± SD was 9.1 ± 1.2 at 37°C compared with 2.4 ± 1.8 at 32°C, 2.3 ± 0.4 at 34°C, and 0.4 ± 0.1 at 40°C (P < 0.01). Changes in PMR were not significant after 2-hour exposure at temperatures ranging from 32°C to 40°C. Morphological changes in parasite cytoplasm and nucleus could be observed after long exposure to low or high temperature. After 48-hour incubation, rosette formation (3 2 uninfected red blood cells bound to infected red blood cells) was decreased at 34°C or 39°C compared with that at 37°C. In conclusion, both hyper- and hypothermia reduce PMR and delay erythrocytic stage development of P. falciparum, subsequently reducing rosette formation.