Publication: Small plaque size variant of chikungunya primary isolate showed reduced virulence in mice
Issued Date
2018-09-01
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ISSN
22288694
0125877X
0125877X
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2-s2.0-85054449439
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.36, No.3 (2018), 201-205
Suggested Citation
Thitigun Jaimipak, Sutee Yoksan, Sukathida Ubol, Rojjanaporn Pulmanausahakul Small plaque size variant of chikungunya primary isolate showed reduced virulence in mice. Asian Pacific Journal of Allergy and Immunology. Vol.36, No.3 (2018), 201-205. doi:10.12932/AP-250417-0079 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45968
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Title
Small plaque size variant of chikungunya primary isolate showed reduced virulence in mice
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Abstract
© 2018, Allergy and Immunology Society of Thailand. All rights reserved. Background: Plaque size is a common feature of viral characterization. Small plaque size is used as a marker of attenuation for live-attenuated vaccine development. Objective: To investigate whether the naturally occurring plaque size variation reflects virulence of the variants of chikungunya virus (CHIKV). Methods: We selected and purified a variant with small plaque size from the primary isolate. The viral variant was tested for the plaque morphology, in vitro growth kinetics and mouse neurovirulence in comparison with the parental wild type. Results: The small plaque size variant showed stable homogenous small plaques after 4 plaque purifications. The small plaque virus grew slower and to the lower titer when compared with wild type virus. After 21 days of infection, mice that received small plaque virus showed 98% survival rate while 74% of mice survived after infected with wild type virus. Conclusion: The small plaque size variant of CHIKV can be obtained by plaque purification and the virus displays decreased virulence.