Publication: Gel-filtration chromatography of Salmonella protein antigens and their implication in immunodiagnosis.
Issued Date
1987-12-01
Resource Type
ISSN
0125877X
Other identifier(s)
2-s2.0-0023463871
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.5, No.2 (1987), 109-117
Suggested Citation
S. Kanjanahareutai, S. Sarasombath, S. Sirisinha Gel-filtration chromatography of Salmonella protein antigens and their implication in immunodiagnosis.. Asian Pacific Journal of Allergy and Immunology. Vol.5, No.2 (1987), 109-117. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15368
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Title
Gel-filtration chromatography of Salmonella protein antigens and their implication in immunodiagnosis.
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Abstract
Crude Barber protein (Bp) antigens were prepared from Salmonella typhi, S. krefeld and S. derby by an original method that has been described previously. These antigens were subjected to gel-filtration chromatography using Sephadex G-200. A sharp peak that eluted together with the void volume was thus separated from a broad second peak that eluted from the column at positions equivalent to 118,000 to 12,000 daltons. The proteins eluted in the latter peak were arbitrarily divided into 5 fractions and, together with the first peak, subjected to polyacrylamide gel electrophoresis and immunoprecipitation with both homologous and heterologous rabbit antisera. The extent of immunological cross reactivities was determined by enzyme-linked immunosorbent assay. The preliminary results obtained by this technique showed species-specific protein antigens to have molecular weights ranging between 36,000 and 68,000 daltons.