Publication:
Characterization of a ferric uptake regulator (fur) gene from Xanthomonas campestris pv. phaseoli with unusual primary structure, genome organization, and expression patterns

1

Issued Date

1999-11-01

Resource Type

Article

ISSN

03781119

DOI

10.1016/S0378-1119(99)00412-6

Other identifier(s)

2-s2.0-0032733344

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Gene. Vol.239, No.2 (1999), 251-258

Suggested Citation

Suvit Loprasert, Ratiboot Sallabhan, Sopapan Atichartpongkul, Skorn Mongkolsuk Characterization of a ferric uptake regulator (fur) gene from Xanthomonas campestris pv. phaseoli with unusual primary structure, genome organization, and expression patterns. Gene. Vol.239, No.2 (1999), 251-258. doi:10.1016/S0378-1119(99)00412-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/25327

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

Characterization of a ferric uptake regulator (fur) gene from Xanthomonas campestris pv. phaseoli with unusual primary structure, genome organization, and expression patterns

Author(s)

Suvit Loprasert
 Ratiboot Sallabhan
 Sopapan Atichartpongkul
 Skorn Mongkolsuk

Other Contributor(s)

Chulabhorn Research Institute
 Mahidol University

Abstract

A 1.5kb DNA fragment from Xanthomonas campestris pv. phaseoli containing fur was characterized. fur is a single copy gene that is transcribed as a monocistronic mRNA. The predicted amino acid sequence of Xp Fur showed extensive identity to other Fur proteins. However, Xp Fur has many distinct features, particularly a lack of cysteine residues in the conserved metal- binding motifs and unusual modifications in the carboxy-terminus region. The nucleotide sequences of fur genes from four other Xanthomonas spp. were determined. Deduced amino acid sequences all showed the distinct features of Xp Fur. Functionally, Xp Fur partially repressed a Fur-regulated promoter in E. coli. Expression analysis of fur showed increased fur mRNA levels in response to a low iron growth condition. The fur transcription start site was identified by primer extension.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/25327

Collections

Scopus 1991-2000