Publication: Genomic organization and putative promoters of highly conserved glutathione S-transferases originating by alternative splicing in Anopheles dirus
2
Issued Date
2001-01-01
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ISSN
09651748
Other identifier(s)
2-s2.0-0035188116
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Mahidol University
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SCOPUS
Bibliographic Citation
Insect Biochemistry and Molecular Biology. Vol.31, No.1 (2001), 75-85
Suggested Citation
Saengtong Pongjaroenkit, Kanya Jirajaroenrat, Chanikarn Boonchauy, Umnaj Chanama, Somphob Leetachewa, La aied Prapanthadara, Albert J. Ketterman Genomic organization and putative promoters of highly conserved glutathione S-transferases originating by alternative splicing in Anopheles dirus. Insect Biochemistry and Molecular Biology. Vol.31, No.1 (2001), 75-85. doi:10.1016/S0965-1748(00)00107-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/26416
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Title
Genomic organization and putative promoters of highly conserved glutathione S-transferases originating by alternative splicing in Anopheles dirus
Abstract
The genomic DNA of a GST class I alternative splicing gene has been characterized from Anopheles dirus, a Thai malaria vector. This gene organization is highly conserved in An. dirus and Anopheles gambiae (aggstlα), with >80% nucleotide identity in the coding region. Their gene organization contains six exons for four mature GST transcripts, which share exon 1 and exon 2 but vary between four different exon 3 sequences (exon 3A-3D). The deduced amino acid sequence of the GST transcripts from these two genes also shows very high conservation, with 85-93% identity for each orthologous gene. Two putative promoters and possible regulatory elements were predicted by a combination of the TSSW and MatInspector programs. The Ad214 promoter is proposed to be involved in developmental stage regulation. The Ad2112 promoter would appear to respond to intra- or extracellular stimuli. These two Anopheline species appear to have diverged in the distant past based on gene neighbors and phylogenetic data, yet these GST genes are still conserved. Therefore it is highly probable that this GST gene organization has one or more important roles. © 2001 Elsevier Science Ltd.