Publication: Quercetin inhibits colorectal cancer cells induced-angiogenesis in both colorectal cancer cell and endothelial cell through downregulation of vegf-a/vegfr2
Issued Date
2021-06-01
Resource Type
ISSN
22180532
00368709
00368709
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2-s2.0-85107729260
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Mahidol University
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SCOPUS
Bibliographic Citation
Scientia Pharmaceutica. Vol.89, No.2 (2021)
Suggested Citation
Tamonwan Uttarawichien, Chantra Kamnerdnond, Tasanee Inwisai, Prasit Suwannalert, Nathawut Sibmooh, Witchuda Payuhakrit Quercetin inhibits colorectal cancer cells induced-angiogenesis in both colorectal cancer cell and endothelial cell through downregulation of vegf-a/vegfr2. Scientia Pharmaceutica. Vol.89, No.2 (2021). doi:10.3390/scipharm89020023 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/78956
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Title
Quercetin inhibits colorectal cancer cells induced-angiogenesis in both colorectal cancer cell and endothelial cell through downregulation of vegf-a/vegfr2
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Abstract
Colorectal cancer (CRC) aggressiveness is caused by cancer angiogenesis which promotes the cancer growth and metastasis associated with poor prognosis and poor survival. The vascular endothelial growth factor-A (VEGF-A) and its receptor (VEGFR-2) form the major signaling pathway in cancer angiogenesis. This study aimed to investigate the anti-angiogenesis activity of quercetin in both colorectal cancer cells and endothelial cells. The tube formation of human vein endothelial cells (HUVECs) was determined by using conditioned media of HT-29 cells treated with quercetin co-cultured with HUVECs. The VEGF-A and NF-κB p65 protein expressions in the quercetin-treated HT-29 cells were determined by fluorescence assay and Western blot analysis. The VEGFR-2 protein expression in HUVECs was determined after they were co-cultured with the quercetin-treated HT-29 cells. Quercetin markedly decreased the HT-29 cell-induced angiogenesis in HUVECs. NF-κB p65 and VEGF-A protein expression were also inhibited by quercetin. Moreover, quercetin significantly inhibited VEGFR-2 expression and translocation in HUVECs after they were co-cultured with high dose quercetin-treated HT-29 cells. Taken together, quercetin had an anti-angiogenesis effect on VEGF-A inhibition related to the NF-κB signaling pathway in the HT-29 cells and reduced VEGFR-2 expression and translocation in HUVECs.