Publication: Diversity of the 47-kD HtrA nucleic acid and translated amino acid sequences from 17 recent human isolates of Orientia
Issued Date
2013-06-10
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ISSN
15577759
15303667
15303667
Other identifier(s)
2-s2.0-84878556148
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Mahidol University
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SCOPUS
Bibliographic Citation
Vector-Borne and Zoonotic Diseases. Vol.13, No.6 (2013), 367-375
Suggested Citation
Ju Jiang, Daniel H. Paris, Stuart D. Blacksell, Nuntipa Aukkanit, Paul N. Newton, Rattanaphone Phetsouvanh, Leonard Izzard, John Stenos, Stephen R. Graves, Nicholas P.J. Day, Allen L. Richards Diversity of the 47-kD HtrA nucleic acid and translated amino acid sequences from 17 recent human isolates of Orientia. Vector-Borne and Zoonotic Diseases. Vol.13, No.6 (2013), 367-375. doi:10.1089/vbz.2012.1112 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31906
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Title
Diversity of the 47-kD HtrA nucleic acid and translated amino acid sequences from 17 recent human isolates of Orientia
Abstract
Orientia tsutsugamushi, the etiologic agent of potentially fatal scrub typhus, is characterized by a high antigenic diversity, which complicates the development of a broadly protective vaccine. Efficacy studies in murine and nonhuman primate models demonstrated the DNA vaccine candidate pKarp47, based upon the O. tsutsugamushi Karp 47-kD HtrA protein gene, to be a successful immunoprophylactic against scrub typhus. To characterize 47-kD HtrA protein diversity among human isolates of Orientia, we sequenced the full open reading frame (ORF) of the 47-kD HtrA gene and analyzed the translated amino acid sequences of 17 patient isolates from Thailand (n=13), Laos (n=2), Australia (n=1), and the United Arab Emirates (UAE) (n=1) and 9 reference strains: Karp (New Guinea), Kato (Japan), Ikeda (Japan), Gilliam (Burma), Boryong (Korea), TA763, TH1811 and TH1817 (Thailand), and MAK243 (China). The percentage identity (similarity) of translated amino acid sequences between 16 new isolates and 9 reference strains of O. tsutsugamushi ranged from 96.4% to 100% (97.4% to 100%). However, inclusion of the recently identified Orientia chuto sp. nov. reduced identity (similarity) values to 82.2% to 83.3% (90.4% to 91.4%). These results demonstrate the diversity of Orientia 47-kD HtrA among isolates encountered by humans and therefore provide support for the necessity of developing a broadly protective scrub typhus vaccine that takes this diversity into account. © Copyright 2013, Mary Ann Liebert, Inc.