Publication: Proteome and allergenome of Asian wasp, Vespa affinis, venom and IgE reactivity of the venom components
Issued Date
2014-03-07
Resource Type
ISSN
15353907
15353893
15353893
Other identifier(s)
2-s2.0-84896760156
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Proteome Research. Vol.13, No.3 (2014), 1336-1344
Suggested Citation
Nitat Sookrung, Siriporn Wong-Din-Dam, Anchalee Tungtrongchitr, Onrapak Reamtong, Nitaya Indrawattana, Yuwaporn Sakolvaree, Nualanong Visitsunthorn, Wiparat Manuyakorn, Wanpen Chaicumpa Proteome and allergenome of Asian wasp, Vespa affinis, venom and IgE reactivity of the venom components. Journal of Proteome Research. Vol.13, No.3 (2014), 1336-1344. doi:10.1021/pr4009139 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33289
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Proteome and allergenome of Asian wasp, Vespa affinis, venom and IgE reactivity of the venom components
Other Contributor(s)
Abstract
Vespa affinis (Asian wasp, Thai banded tiger wasp, or local name: Tor Hua Seua) causes the most frequent incidence of medically important Hymenoptera sting in South and Southeast Asia. However, data on the venom components attributable to the sting derived-clinical manifestations (local reactions, IgE mediated-anaphylaxis, or systemic envenomation) are lacking. This study provides the first set information on V. affinis venom proteome, allergenome, and IgE reactivity of individual venom components. From 2DE-gel based-proteomics, the venom revealed 93 protein spots, of which proteins in 51 spots could be identified and classified into three groups: typical venom components and structural and housekeeping proteins. Venom proteins in 32 spots reacted with serum IgE of wasp allergic patients. Major allergenic proteins that reacted to IgE of >50% of the wasp allergic patients included PLA1 (100%), arginine kinase (73%), heat shock 70 kDa protein (73.3%), venom allergen-5 (66.7%), enolase (66.7%), PLA1 magnifin (60%), glyceraldehyde-3- phosphate dehydrogenase (60%), hyaluronidase (53.3%), and fructose-bisphosphate aldolase (53.3%). The venom minor allergens were GB17876 transcript (40%), GB17291 transcript (20%), malic enzyme (13.3%), aconitate hydratase (6.7%), and phosphoglucomutase (6.7%). The information has diagnostic and clinical implications for future improvement of case diagnostic sensitivity and specificity, component-resolve diagnosis, and design of specific Hymenoptera venom immunotherapy. © 2014 American Chemical Society.