Publication: Genes for hydrogen peroxide detoxification and adaptation contribute to protection against heat shock in Xanthomonas campestrispv. campestris
1
Issued Date
2011-04-01
Resource Type
ISSN
15746968
03781097
03781097
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2-s2.0-79952128487
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Mahidol University
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SCOPUS
Bibliographic Citation
FEMS Microbiology Letters. Vol.317, No.1 (2011), 60-66
Suggested Citation
Sarinya Buranajitpakorn, Anong Piwkam, Nisanart Charoenlap, Paiboon Vattanaviboon, Skorn Mongkolsuk Genes for hydrogen peroxide detoxification and adaptation contribute to protection against heat shock in Xanthomonas campestrispv. campestris. FEMS Microbiology Letters. Vol.317, No.1 (2011), 60-66. doi:10.1111/j.1574-6968.2011.02211.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11570
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Title
Genes for hydrogen peroxide detoxification and adaptation contribute to protection against heat shock in Xanthomonas campestrispv. campestris
Abstract
Xanthomonas campestris pv. campestris, a soil-borne plant-pathogenic bacterium, is exposed to multiple stresses in the environment and during interaction with a host plant. The roles of hydrogen peroxide (H 2 O 2 )-protective genes (katA, katG, and ahpC) and a peroxide sensor/transcription regulator (oxyR) in the viability of X. campestris pv. campestris at an elevated temperature were evaluated. The single katA and katG mutants showed moderate decreased survival after the heat treatment, while the double katA-katG and oxyR mutants were the most vulnerable to the heat treatment compared with a wild-type strain. However, ahpC provided no protective function against the heat treatment. Flow cytometric analysis revealed an increased accumulation of peroxide in cells treated with heat. Altogether, the data revealed a crucial role of genes in the H 2 O 2 detoxification system for protection against lethal heat shock in X. campestris pv. campestris. © 2011 Federation of European Microbiological Societies.