Publication:
Human T cell responses to Japanese encephalitis virus in health and disease

Issued Date

2016-01-01

Resource Type

Article

ISSN

15409538
00221007

DOI

10.1084/jem.20151517

Other identifier(s)

2-s2.0-84977658045

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Experimental Medicine. Vol.213, No.7 (2016), 1331-1352

Suggested Citation

Lance Turtle, Tanushka Bali, Gemma Buxton, Savita Chib, Sajesh Chan, Mohammed Soni, Mohammed Hussain, Heather Isenman, Prachi Fadnis, Manjunatha M. Venkataswamy, Vishali Satishkumar, Penny Lewthwaite, Ayako Kurioka, Srinivasa Krishna, M. Veera Shankar, Riyaz Ahmed, Ashia Begum, Vasanthapuram Ravi, Anita Desai, Sutee Yoksan, Stefan Fernandez, Christian B. Willberg, Henrik N. Kloverpris, Christopher Conlon, Paul Klenerman, Vijaya Satchidanandam, Tom Solomon Human T cell responses to Japanese encephalitis virus in health and disease. Journal of Experimental Medicine. Vol.213, No.7 (2016), 1331-1352. doi:10.1084/jem.20151517 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40901

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Title

Human T cell responses to Japanese encephalitis virus in health and disease

Author(s)

Lance Turtle
 Tanushka Bali
 Gemma Buxton
 Savita Chib
 Sajesh Chan
 Mohammed Soni
 Mohammed Hussain
 Heather Isenman
 Prachi Fadnis
 Manjunatha M. Venkataswamy
 Vishali Satishkumar
 Penny Lewthwaite
 Ayako Kurioka
 Srinivasa Krishna
 M. Veera Shankar
 Riyaz Ahmed
 Ashia Begum
 Vasanthapuram Ravi
 Anita Desai
 Sutee Yoksan
 Stefan Fernandez
 Christian B. Willberg
 Henrik N. Kloverpris
 Christopher Conlon
 Paul Klenerman
 Vijaya Satchidanandam
 Tom Solomon

Other Contributor(s)

University of Liverpool
 Royal Liverpool and Broadgreen University Hospitals NHS Trust
 Indian Institute of Science, Bangalore
 Vijayanagar Institute of Medical Sciences
 National Institute of Mental Health and Neuro Sciences
 National Health Service
 University of Oxford
 Mahidol University
 Armed Forces Research Institute of Medical Sciences, Thailand

Abstract

© 2016 Turtle et al. Japanese encephalitis (JE) virus (JEV) is an important cause of encephalitis in children of South and Southeast Asia. However, the majority of individuals exposed to JEV only develop mild symptoms associated with long-lasting adaptive immunity. The related flavivirus dengue virus (DENV) cocirculates in many JEV-endemic areas, and clinical data suggest cross-protection between DENV and JEV. To address the role of T cell responses in protection against JEV, we conducted the first full-breadth analysis of the human memory T cell response using a synthetic peptide library. Ex vivo interferon-γ (IFN-γ) responses to JEV in healthy JEV-exposed donors were mostly CD8+and targeted nonstructural (NS) proteins, whereas IFN-γ responses in recovered JE patients were mostly CD4+and targeted structural proteins and the secreted protein NS1. Among patients, a high quality, polyfunctional CD4+T cell response was associated with complete recovery from JE. T cell responses from healthy donors showed a high degree of cross-reactivity to DENV that was less apparent in recovered JE patients despite equal exposure. These data reveal divergent functional CD4+and CD8+T cell responses linked to different clinical outcomes of JEV infection, associated with distinct targeting and broad flavivirus cross-reactivity including epitopes from DENV, West Nile, and Zika virus.

Keyword(s)

Immunology and Microbiology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/40901

Collections

Scopus 2016-2017