Publication: Antigenic components, isolation and partial characterization of excretion-secretion fraction of Paramphistomum cervi
Issued Date
2013-03-01
Resource Type
ISSN
10902449
00144894
00144894
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2-s2.0-84872542008
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.133, No.3 (2013), 327-333
Suggested Citation
Panat Anuracpreeda, Jaruwan Poljaroen, Charoonroj Chotwiwatthanakun, Yotsawan Tinikul, Prasert Sobhon Antigenic components, isolation and partial characterization of excretion-secretion fraction of Paramphistomum cervi. Experimental Parasitology. Vol.133, No.3 (2013), 327-333. doi:10.1016/j.exppara.2012.12.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31951
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Title
Antigenic components, isolation and partial characterization of excretion-secretion fraction of Paramphistomum cervi
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Abstract
The immunogenic components of adult Paramphistomum cervi excretion-secretion (ES) fraction were revealed by SDS-PAGE and immunoblotting technique using sera from cattle naturally infected with P. cervi, Fasciola gigantica, strongylids, Trichuris sp., and Strongyloides sp. By SDS-PAGE, it was found that the ES fraction comprised 13 distinct protein bands. Immunoblotting analysis of these proteins exhibited nine prominent antigenic bands which were recognized by paramphistomosis antisera. These antigenic proteins had molecular weights ranging from 10-170. kDa. One antigenic protein band of 40. kDa was found to give a consistent reaction with sera from all infected cattle. Its diagnostic sensitivity, specificity and accuracy using this test were 100%, 98.9% and 99.3%, respectively. The positive and negative predictive values were 98% and 100%, respectively. The 40. kDa antigen was partially purified by gel filtration and ion-exchange chromatography. The antigenicity of 40. kDa protein for diagnosis of P. cervi infection was confirmed by immunoblotting and indirect ELISA (at 1:78,125 dilution) using a pool of sera and individual serum samples from infected cattle. The present findings suggest that the 40. kDa protein may be used as a diagnostic antigen for paramphistomosis. © 2012 Elsevier Inc.