Publication: Cloning and characterization of a mosquito larvicidal toxin produced during vegetative stage of Bacillus sphaericus 2297
Issued Date
2004-01-01
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ISSN
03438651
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2-s2.0-3242750630
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Mahidol University
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SCOPUS
Bibliographic Citation
Current Microbiology. Vol.49, No.2 (2004), 84-88
Suggested Citation
Boonhiang Promdonkoy, Patcharee Promdonkoy, Sutipa Tanapongpipat, Plearnpis Luxananil, Namchai Chewawiwat, Mongkon Audtho, Sakol Panyim Cloning and characterization of a mosquito larvicidal toxin produced during vegetative stage of Bacillus sphaericus 2297. Current Microbiology. Vol.49, No.2 (2004), 84-88. doi:10.1007/s00284-004-4274-y Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21413
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Title
Cloning and characterization of a mosquito larvicidal toxin produced during vegetative stage of Bacillus sphaericus 2297
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Abstract
The mosquitocidal toxin 1 (mtx1) gene from genomic DNA of B. sphaericus strain 2297 was cloned and expressed in E. coli. DNA sequencing analysis of the cloned gene revealed a single open reading frame encoding an 870-amino acid polypeptide. Expression level of the full-length gene in E. coli was very low even though strong promoter was used or the gene was expressed as a fusion protein. Expression level was highly improved after the putative leader sequence was deleted, and the truncated gene was expressed as a fusion protein with glutathione S-transferase (GST-tMtx1). E. coli cells expressing GST-tMtx1 was highly toxic to Culex quinquefasciatus larvae and showed lower toxicity against Anopheles dirus and Aedes aegypti larvae. Enterobacter amnigenus An11, a mosquito larval gut colonizable bacteria, transformed with the cloned gene exhibited mosquito larvicidal activity. Result suggested that there is a potential to develop this protein to be used as an alternative mosquito control agent.