Publication: Identification and expression of prostaglandin E synthase (PGES) gene in the central nervous system and ovary during ovarian maturation of the female mud crab, Scylla olivacea
Issued Date
2018-11-01
Resource Type
ISSN
03784320
Other identifier(s)
2-s2.0-85054138204
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Animal Reproduction Science. Vol.198, (2018), 220-232
Suggested Citation
Supawadee Duangprom, Wilailuk Ampansri, Saowaros Suwansa-ard, Charoonroj Chotwiwatthanakun, Prasert Sobhon, Napamanee Kornthong Identification and expression of prostaglandin E synthase (PGES) gene in the central nervous system and ovary during ovarian maturation of the female mud crab, Scylla olivacea. Animal Reproduction Science. Vol.198, (2018), 220-232. doi:10.1016/j.anireprosci.2018.09.022 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/44656
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Identification and expression of prostaglandin E synthase (PGES) gene in the central nervous system and ovary during ovarian maturation of the female mud crab, Scylla olivacea
Other Contributor(s)
Abstract
© 2018 Elsevier B.V. Prostaglandins have important physiological roles in marine invertebrates, including larval development and reproduction. The prostaglandin E concentration fluctuates during the ovarian development of crustaceans. The biosynthetic pathway of prostaglandin, however, has not been well studied in portunid crabs, including in the mud crab, Scylla olivacea. In this study, the aim was to investigate the presence of prostaglandin E synthase (PGES), enzyme that catalyzes the terminal conversion in the prostaglandin E2 (PGE 2 ) biosynthesis, and its gene expression in the central nervous system (CNS) and ovary during ovarian maturation of S. olivacea. cDNA sequence encoding PGES was cloned from the S. olivacea ovary. The PGES transcript of S. olivacea (Scyol-PGES) consists of 1258 nucleotides, which encodes for 420 amino acid PGES protein precursor. Investigation of gene expression by RT-PCR indicated that Scyol-PGES was detected in all organs studied. Based on in situ hybridization, Scyol-PGES was detected in the I to III stages for oocyte development of Stage 3 of ovarian development, and in the CNS, including the various neuronal clusters of the brain. In the ventral nerve cord, the Scyol-PGES gene was expressed in the neurons within the subesophageal, thoracic and abdominal ganglia. The Scyol-PGES gene expression as indicated by relative abundance of mRNA in the Stage 4 of ovarian development was greater than that at Stages 1 to 3 of ovarian development. This is the first report on PGES in the mud crab, S. olivacea, and its gene expression suggested the involvement of PGES in the ovarian development of this species.