Publication: In vitro inhibitory effect of cytochalasins on intestinal phenylalanine absorption in the mouse
Issued Date
1983-01-01
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ISSN
00296635
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2-s2.0-0020614089
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Mahidol University
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SCOPUS
Bibliographic Citation
Nutrition Reports International. Vol.27, No.3 (1983), 611-618
Suggested Citation
C. Toskulkao, T. Glinsukon, W. Wangpanish In vitro inhibitory effect of cytochalasins on intestinal phenylalanine absorption in the mouse. Nutrition Reports International. Vol.27, No.3 (1983), 611-618. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30427
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Title
In vitro inhibitory effect of cytochalasins on intestinal phenylalanine absorption in the mouse
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Abstract
Inhibition of phenylalanine absorption by several cytochalasins was studied in the everted sacs of mouse jejunum in vitro. Five cytochalasins (A, B, C, D, and E) at a concentration of 10.0 μg/ml highly inhibited Na+-dependent phenylalanine absorption by 13.1 to 47.1%. Cytochalasin E appeared to be a most potent congener among other cytochalasins and it caused an inhibition of phenylalanine absorption by 32.6% at low concentration of 2.5 μg/ml. In the kinetic study, cytochalasin E induced an alteration of both K(m) (1.4 x 10-3to 2.3 x 10-3M) and V(max) (59.8 to 14.6 μmoles/g wet wt/60 min) values. It is suggested therefore that cytochalasin E may probably inhibit the absorption of actively transported amino acid in part by interfering with the binding sites of carriers on microvillar membrane.