Publication:
Antimicrobial effect of asiatic acid against Clostridium difficile is associated with disruption of membrane permeability

dc.contributor.authorPhurt Harnvoravongchaien_US
dc.contributor.authorSurang Chankhamhaengdechaen_US
dc.contributor.authorPuey Ounjaien_US
dc.contributor.authorSombat Singhakaewen_US
dc.contributor.authorKanpong Boonthawornen_US
dc.contributor.authorTavan Janvilisrien_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2019-08-23T11:17:54Z
dc.date.available2019-08-23T11:17:54Z
dc.date.issued2018-09-07en_US
dc.description.abstract© 2018 Harnvoravongchai, Chankhamhaengdecha, Ounjai, Singhakaew, Boonthaworn and Janvilisri. Antibiotic resistance is a major concern in Clostridium difficile, the causative agent of antibiotic-associated diarrhea. Reduced susceptibility to first- and second-line agents is widespread, therefore various attempts have been made to seek alternative preventive and therapeutic strategies against this pathogen. In this work, the antimicrobial properties of asiatic acid were evaluated against C. difficile. Asiatic acid displayed substantial inhibitory effects on 19 C. difficile isolates collected from different sources with minimal inhibitory concentrations ranging from 10 to 20 μg/ml. Time kill analysis and minimal bactericidal concentration revealed potential bactericidal activity of this compound. Asiatic acid induced membrane damages and alterations in morphological ultrastructure in C. difficile, thereby causing the leakage of intracellular substances. Moreover, asiatic acid also displayed an inhibitory effect on cell motility, but did not interfere with biofilm formation and spore germination. Analysis of drug combination showed no synergistic effect between asiatic acid and vancomycin/metronidazole. Altogether, asiatic acid exhibited strong antimicrobial activity against vegetative cells and could serve as an alternative resource for tackling C. difficile.en_US
dc.identifier.citationFrontiers in Microbiology. Vol.9, No.SEP (2018)en_US
dc.identifier.doi10.3389/fmicb.2018.02125en_US
dc.identifier.issn1664302Xen_US
dc.identifier.other2-s2.0-85053067884en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/45966
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053067884&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleAntimicrobial effect of asiatic acid against Clostridium difficile is associated with disruption of membrane permeabilityen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053067884&origin=inwarden_US

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