Publication: Development of polymorphic markers from expressed sequence tags of Manihot esculenta Crantz
Issued Date
2008-05-01
Resource Type
ISSN
1755098X
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2-s2.0-43949124804
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular Ecology Resources. Vol.8, No.3 (2008), 682-685
Suggested Citation
S. Tangphatsornruang, S. Sraphet, R. Singh, E. Okogbenin, M. Fregene, K. Triwitayakorn Development of polymorphic markers from expressed sequence tags of Manihot esculenta Crantz. Molecular Ecology Resources. Vol.8, No.3 (2008), 682-685. doi:10.1111/j.1471-8286.2007.02047.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18743
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Title
Development of polymorphic markers from expressed sequence tags of Manihot esculenta Crantz
Abstract
In this study, 49 primers were designed from sequences containing di-, tri-, tetra-, penta- and hexanucleotide motifs with a minimum of four repeats and presence of motif size polymorphisms (insertion/deletion) from cassava (Manihot esculenta Crantz) expressed sequence tags deposited in public sequence database. Each locus was subsequently screened on 29 M. esculenta Crantz obtained from 15 different countries. Cross-amplification was tested with M. esculenta Crantz (ssp. flabellifolia) and four different Manihot species, M. chlorosticta, M. carthaginensis, M. filamentosa and M. tristis. Of these, nine loci showed polymorphic profiles within M. esculenta Crantz, which revealed two to four alleles per locus. The average unbiased and direct count heterozygosities were 0.4901 and 0.5674, respectively. © 2007 The Authors.