Publication: N-linked glycosylation of dengue virus NS1 protein modulates secretion, cell-surface expression, hexamer stability, and interactions with human complement
Issued Date
2011-05-10
Resource Type
ISSN
10960341
00426822
00426822
Other identifier(s)
2-s2.0-79954633991
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Virology. Vol.413, No.2 (2011), 253-264
Suggested Citation
Pawit Somnuke, Richard E. Hauhart, John P. Atkinson, Michael S. Diamond, Panisadee Avirutnan N-linked glycosylation of dengue virus NS1 protein modulates secretion, cell-surface expression, hexamer stability, and interactions with human complement. Virology. Vol.413, No.2 (2011), 253-264. doi:10.1016/j.virol.2011.02.022 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12048
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
N-linked glycosylation of dengue virus NS1 protein modulates secretion, cell-surface expression, hexamer stability, and interactions with human complement
Other Contributor(s)
Abstract
Dengue virus (DENV) NS1 is a versatile non-structural glycoprotein that is secreted as a hexamer, binds to the cell surface of infected and uninfected cells, and has immune evasive functions. DENV NS1 displays two conserved N-linked glycans at N130 and N207. In this study, we examined the role of these two N-linked glycans on NS1 secretion, stability, and function. Because some groups have reported reduced yields of infectious DENV when N130 and N207 are changed, we analyzed glycosylation-deficient NS1 phenotypes using a transgenic expression system. We show that the N-linked glycan at position 130 is required for stabilization of the secreted hexamer whereas the N-linked glycan at residue 207 facilitates secretion and extracellular protein stability. Moreover, NS1 mutan ts lacking an N-linked glycan at N130 did not interact efficiently with complement components C1s and C4. In summary, our results elucidate the contribution of N-linked glycosylation to the function of DENV NS1. © 2011 Elsevier Inc.