Publication: Quantification of artemisinin in human plasma using liquid chromatography coupled to tandem mass spectrometry
Issued Date
2009-04-05
Resource Type
ISSN
07317085
Other identifier(s)
2-s2.0-61449245434
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.49, No.3 (2009), 768-773
Suggested Citation
N. Lindegardh, J. Tarning, P. V. Toi, T. T. Hien, J. Farrar, P. Singhasivanon, N. J. White, M. Ashton, N. P J Day Quantification of artemisinin in human plasma using liquid chromatography coupled to tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. Vol.49, No.3 (2009), 768-773. doi:10.1016/j.jpba.2008.12.014 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27246
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Title
Quantification of artemisinin in human plasma using liquid chromatography coupled to tandem mass spectrometry
Abstract
A liquid chromatographic tandem mass spectroscopy method for the quantification of artemisinin in human heparinised plasma has been developed and validated. The method uses Oasis HLB™ μ-elution solid phase extraction 96-well plates to facilitate a high throughput of 192 samples a day. Artesunate (internal standard) in a plasma-water solution was added to plasma (50 μL) before solid phase extraction. Artemisinin and its internal standard artesunate were analysed by liquid chromatography and MS/MS detection on a Hypersil Gold C18 (100 mm × 2.1 mm, 5 μm) column using a mobile phase containing acetonitrile-ammonium acetate 10 mM pH 3.5 (50:50, v/v) at a flow rate of 0.5 mL/min. The method has been validated according to published FDA guidelines and showed excellent performance. The within-day, between-day and total precisions expressed as R.S.D., were lower than 8% at all tested quality control levels including the upper and lower limit of quantification. The limit of detection was 0.257 ng/mL for artemisinin and the calibration range was 1.03-762 ng/mL using 50 μL plasma. The method was free from matrix effects as demonstrated both graphically and quantitatively. © 2008 Elsevier B.V. All rights reserved.