Publication: Genotypic resistance profiles in antiretroviral-naive HIV-1 infections before and after initiation of first-line HAART: impact of polymorphism on resistance to therapy
Issued Date
2008-03-01
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ISSN
09248579
Other identifier(s)
2-s2.0-39149129052
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Antimicrobial Agents. Vol.31, No.3 (2008), 277-281
Suggested Citation
Chonlaphat Sukasem, Vina Churdboonchart, Wisut Sukeepaisarncharoen, Wantanich Piroj, Tasanee Inwisai, Montip Tiensuwan, Wasun Chantratita Genotypic resistance profiles in antiretroviral-naive HIV-1 infections before and after initiation of first-line HAART: impact of polymorphism on resistance to therapy. International Journal of Antimicrobial Agents. Vol.31, No.3 (2008), 277-281. doi:10.1016/j.ijantimicag.2007.10.029 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19750
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Title
Genotypic resistance profiles in antiretroviral-naive HIV-1 infections before and after initiation of first-line HAART: impact of polymorphism on resistance to therapy
Abstract
Genotypic testing using TRUGENE™ was performed for treatment-naive, human immunodeficiency virus type 1 (HIV-1)-infected patients at baseline and after initiation of protease inhibitor (PI)-based highly active antiretroviral therapy (HAART) regimens. The genetic diversity of HIV-1 pol sequences from 92 CRF01_AE and 21 B strains was compared. Subsequently, the impact of polymorphism on resistance to therapy was studied in CRF01_AE-infected (n = 29) and subtype B-infected (n = 14) patients. At baseline, the differences between CRF01_AE and B strain were mainly observed in the minor mutations L10I/V, M36I and L63P/I/H (P < 0.001, χ2). In the reverse transcriptase sequence, M41L and T215Y/S were more common in patients infected with subtype B virus (P < 0.05, χ2). Although all patients treated with PI-based HAART had pre-existing minor mutations, a low prevalence of resistance to PIs was observed (5/43; 11.6%). Moreover, major mutations (D30N and N88D) conferring resistance to PIs were found in patients infected with subtype B strain. In conclusion, polymorphisms at the protease region may not reduce PI susceptibility during treatment. However, this study also revealed the difference in natural mutations among subtypes, which may affect the manifestation of drug resistance. © 2008.