Publication: Mitochondrial cytochrome b gene in two developmental stages of human malarial parasite Plasmodium falciparum.
Issued Date
1995-12-01
Resource Type
ISSN
01251562
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2-s2.0-0029422421
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Mahidol University
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SCOPUS
Bibliographic Citation
The Southeast Asian journal of tropical medicine and public health. Vol.26, No.4 (1995), 600-605
Suggested Citation
S. Petmitr, J. Krungkrai Mitochondrial cytochrome b gene in two developmental stages of human malarial parasite Plasmodium falciparum.. The Southeast Asian journal of tropical medicine and public health. Vol.26, No.4 (1995), 600-605. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17341
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Title
Mitochondrial cytochrome b gene in two developmental stages of human malarial parasite Plasmodium falciparum.
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Abstract
The cytochrome b gene of the mitochondrial ubiquinol-cytochrome c reductase (complex III of electron transport chain) was characterized in two developmental stages of human malarial parasite cultivated in vitro. The cytochrome b gene spanning the nucleotide position 4691 to 5930 in 6-kb mitochondrial DNA from gametocytic (sexual) and intraerythrocytic (asexual) stages of Plasmodium falciparum (a T9,94 mutant line) were in vitro amplified from total DNA using polymerase chain reaction (PCR). It was found that the parasites from both stages contained the PCR product approximately 1.2 kb in length that was localized in mitochondria. The nucleotide sequences of cytochrome b gene at Qi/quinone binding site from both stages were analyzed using thermal cycle sequencing and were found to be the same. The amount of this gene from both stages of the parasite were determined by using the quantitative PCR method. The results showed that the amount of the cytochrome b gene produced from the sexual stage was seven times higher than that obtained from the asexual stage. Our results would provide basic information on the regulation of cytochrome b and the 6-kb mitochondrial DNA during growth and development of the sexual and asexual stages of the malarial parasite in the mammalian host.