Publication: Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax
Issued Date
2014-01-01
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ISSN
18730329
13835769
13835769
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2-s2.0-84905922588
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology International. Vol.63, No.6 (2014), 777-784
Suggested Citation
Suganya Yongkiettrakul, Wansadaj Jaroenram, Narong Arunrut, Wanwisa Chareanchim, Supicha Pannengpetch, Rungkarn Suebsing, Wansika Kiatpathomchai, Wichai Pornthanakasem, Yongyuth Yuthavong, Darin Kongkasuriyachai Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax. Parasitology International. Vol.63, No.6 (2014), 777-784. doi:10.1016/j.parint.2014.06.004 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/34076
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Title
Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax
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Abstract
Malaria is largely a preventable and curable disease. However, a delay or an inappropriate treatment can result in serious adverse outcomes for patient. Rapid, simple and cost-effective diagnostic tests that can be easily adapted and rapidly scaled-up at the field or community levels are needed. In this study, accelerated detection methods for the Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) dihydrofolate reductase-thymidylate synthase were developed based on the loop-mediated isothermal amplification (LAMP) method. The developed methods included the use of species-specific biotinylated primers to amplify LAMP amplicons, which were then hybridized to specific FITC-labeled DNA probes and visualized on a chromatographic lateral flow dipstick (LFD). The total LAMP-LFD assay time was approximately 1.5. h. The LAMP-LFD assays showed similar detection limit to conventional PCR assay when performed on plasmid DNA carrying the malaria dhfr-ts genes. The LAMP-LFD showed 10 folds higher detection limit than PCR when performed on genomic DNA samples from Pf and Pv parasites. The dhfr-ts LAMP-LFD assays also have the advantages of reduced assay time and easy format for interpretation of results. © 2014 Elsevier Ireland Ltd.