Publication: LC-QTOF-MS/MS based molecular networking approach for the isolation of α-glucosidase inhibitors and virucidal agents from coccinia grandis (L.) voigt
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Issued Date
2021-12-01
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ISSN
23048158
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2-s2.0-85121346291
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Mahidol University
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SCOPUS
Bibliographic Citation
Foods. Vol.10, No.12 (2021)
Suggested Citation
Maharani A. Astiti, Akanitt Jittmittraphap, Pornsawan Leaungwutiwong, Nopporn Chutiwitoonchai, Patcharee Pripdeevech, Chulabhorn Mahidol, Somsak Ruchirawat, Prasat Kittakoop LC-QTOF-MS/MS based molecular networking approach for the isolation of α-glucosidase inhibitors and virucidal agents from coccinia grandis (L.) voigt. Foods. Vol.10, No.12 (2021). doi:10.3390/foods10123041 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/75518
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Title
LC-QTOF-MS/MS based molecular networking approach for the isolation of α-glucosidase inhibitors and virucidal agents from coccinia grandis (L.) voigt
Abstract
Coccinia grandis or ivy gourd is an edible plant. Its leaves and fruits are used as vegetable in many countries. Many works on antidiabetic activity of a crude extract of C. grandis, i.e., in vitro, in vivo, and clinical trials studies, have been reported. Profiles of the antidiabetic compounds were previously proposed by using LC-MS or GC-MS. However, the compounds re-sponsible for antidiabetic activity have rarely been isolated and characterized by analysis of 1D and 2D NMR data. In the present work, UHPLC-ESI-QTOF-MS/MS analysis and GNPS molecular networking were used to guide the isolation of α-glucosidase inhibitors from an extract of C. grandis leaves. Seven flavonoid glycosides including rutin (1), kaempferol 3-O-rutinoside (2) or nicotiflorin, kaempferol 3-O-robinobioside (3), quercetin 3-O-robinobioside (4), quercetin 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside (5) or CTN-986, kaempferol 3-O-β-D-api-furanosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside (6), and kaempferol 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-galactopyranoside (7) were isolated from C. grandis leaves. This is the first report of glycosides containing apiose sugar in the genus Coc-cinia. These glycosides exhibited remarkable α-glucosidase inhibitory activity, being 4.4–10.3 times more potent than acarbose. Moreover, they also displayed virucidal activity against influenza A virus H1N1, as revealed by the ASTM E1053-20 method.