Publication: Human mesenchymal stromal cell sheets induce macrophages predominantly to an anti-inflammatory phenotype
Issued Date
2018-07-01
Resource Type
ISSN
15578534
15473287
15473287
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2-s2.0-85049759299
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cells and Development. Vol.27, No.13 (2018), 922-934
Suggested Citation
Panithi Sukho, Jan Willem Hesselink, Nicole Kops, Jolle Kirpensteijn, Femke Verseijden, Yvonne M. Bastiaansen-Jenniskens Human mesenchymal stromal cell sheets induce macrophages predominantly to an anti-inflammatory phenotype. Stem Cells and Development. Vol.27, No.13 (2018), 922-934. doi:10.1089/scd.2017.0275 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45123
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Title
Human mesenchymal stromal cell sheets induce macrophages predominantly to an anti-inflammatory phenotype
Abstract
© Copyright 2018, Mary Ann Liebert, Inc. 2018. Tissue healing is a highly complex process involving a cascade of biochemical and cellular events. Excessive inflammation can impair the healing response. Previous in vitro studies have shown that mesenchymal stromal cells can modulate macrophage-induced inflammation and, therefore, are promising candidates for cell-based therapies aimed at promoting tissue repair. Recently, cell sheets were introduced as a new method of delivering stromal cells to the repair site. The goal of the current study was to compare the effect of different types of stromal cell sheets on the inflammatory state of macrophages in vitro. We compared the effects of adipose tissue-derived stromal cell (ASC) sheets, bone marrow derived stromal cell (BMSC) sheets, and fibroblast sheets on macrophage functional phenotype using flow cytometric analysis, gene expression, as well as cell sheet protein secretion. This was evaluated with and without inflammatory stimulation. Viability and senescence for the different types of sheet were also evaluated. Macrophages cultured in ASC sheet conditioned medium (CM) displayed a higher fluorescence intensity of the anti-inflammatory CD206 surface marker than when cultured in BMSC sheet CM and expressed more CCL18 and IL1RA than when cultured in fibroblast sheet CM. Moreover, ASC sheets had higher cell viability and less senescent cells than BMSC sheets and fibroblast sheets. Taken together, ASC and BMSC can stimulate the anti-inflammatory macrophage (M2) phenotype to a better extent than fibroblasts. It is suggested that ASC sheets might outperform BMSC sheets in an inflammatory situation since ASC sheet CM induced-macrophages have more M2 characteristics, and ASC in the sheet was more viable.