Publication:
Chimeric dengue type 2 (vaccine strain PDK-53)/dengue type 1 virus as a potential candidate dengue type 1 virus vaccine

dc.contributor.authorClaire Y.H. Huangen_US
dc.contributor.authorSiritorn Butrapeten_US
dc.contributor.authorDennis J. Pierroen_US
dc.contributor.authorGwong Jen J. Changen_US
dc.contributor.authorAnn R. Hunten_US
dc.contributor.authorNatth Bhamarapravatien_US
dc.contributor.authorDuane J. Gubleren_US
dc.contributor.authorRichard M. Kinneyen_US
dc.contributor.otherNational Center for Emerging and Zoonotic Infectious Diseasesen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-09-07T09:12:10Z
dc.date.available2018-09-07T09:12:10Z
dc.date.issued2000-03-22en_US
dc.description.abstractWe constructed chimeric dengue type 2/type 1 (DEN-2/DEN-1) viruses containing the nonstructural genes of DEN-2 16681 virus or its vaccine derivative, strain PDK-53, and the structural genes (encoding capsid protein, premembrane protein, and envelope glycoprotein) of DEN-1 16007 virus or its vaccine derivative, strain PDK-13. We previously reported that attenuation markers of DEN-2 PDK-53 virus were encoded by genetic loci located outside the structural gene region of the PDK-53 virus genome. Chimeric viruses containing the nonstructural genes of DEN-2 PDK-53 virus and the structural genes of the parental DEN-1 16007 virus retained the attenuation markers of small plaque size and temperature sensitivity in LLC-MK2 cells, less efficient replication in C6/36 cells, and attenuation for mice. These chimeric viruses elicited higher mouse neutralizing antibody titers against DEN-1 virus than did the candidate DEN-1 PDK-13 vaccine virus or chimeric DEN-2/DEN-1 viruses containing the structural genes of the PDK-13 virus. Mutations in the envelope protein of DEN-1 PDK-13 virus affected in vitro phenotype and immunogenicity in mice. The current PDK-13 vaccine is the least efficient of the four Mahidol candidate DEN virus vaccines in human trials. The chimeric DEN-2/DEN-1 virus might be a potential DEN-1 virus vaccine candidate. This study indicated that the infectious clones derived from the candidate DEN-2 PDK-53 vaccine are promising attenuated vectors for development of chimeric flavivirus vaccines.en_US
dc.identifier.citationJournal of Virology. Vol.74, No.7 (2000), 3020-3028en_US
dc.identifier.doi10.1128/JVI.74.7.3020-3028.2000en_US
dc.identifier.issn0022538Xen_US
dc.identifier.other2-s2.0-0034096441en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/25989
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034096441&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.titleChimeric dengue type 2 (vaccine strain PDK-53)/dengue type 1 virus as a potential candidate dengue type 1 virus vaccineen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034096441&origin=inwarden_US

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