Publication: Laboratory scale production of<sup>13</sup>C labeled chitosan by fungi absidia coerulea and gongronella butleri grown in solid substrate and submerged fermentation
Issued Date
2011-03-01
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ISSN
01448617
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2-s2.0-79951516561
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Mahidol University
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SCOPUS
Bibliographic Citation
Carbohydrate Polymers. Vol.84, No.2 (2011), 743-750
Suggested Citation
Nitar Nwe, Tetsuya Furuike, Issey Osaka, Hiroki Fujimori, Hideya Kawasaki, Ryuichi Arakawa, Seiichi Tokura, Willem F. Stevens, Seiji Kurozumi, Yoshimori Takamori, Minoru Fukuda, Hiroshi Tamura Laboratory scale production of<sup>13</sup>C labeled chitosan by fungi absidia coerulea and gongronella butleri grown in solid substrate and submerged fermentation. Carbohydrate Polymers. Vol.84, No.2 (2011), 743-750. doi:10.1016/j.carbpol.2010.06.023 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11728
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Title
Laboratory scale production of<sup>13</sup>C labeled chitosan by fungi absidia coerulea and gongronella butleri grown in solid substrate and submerged fermentation
Abstract
Nowadays, chitin and chitosan are applied in many medical and pharmaceutical products. However little is known about the metabolism of chitin and chitosan in vivo. In the human body, lysozyme will degrade chitin and chitosan into chito-oligosaccharides. 13 C labeled chitosan is an essential prerequisite for the further study of the fate of chito- oligosaccharides in vivo. To fulfill this requirement, chitosans were extracted from mycelia of fungi, Absidia coerulea ATCC 14076 and Gongronella butleri USDB 0201 and ATCC 42618 grown in solid substrate fermentation (SSF) and submerged fermentation (SMF) to select the best fungus and fermentation method for the production of 13 C labeled chitosan. Based on the production yield of chitosan, the SSF method is the best method for the production of fungal chitosan when compared with SMF methods (i.e., fed-batch fermentation and batch fermentation). However synthesis of 13 C labeled chitosan in cell wall of G. butleri grown in SSF medium coated with 1- 13 C-glucose was not observed. Alternatively, fungus A. coerulea was grown in SMF medium containing 2- 13 C-glucose. The successful synthesis of 13 C labeled glucosamine from 2- 13 C-glucose was observed in mycelia of A. coerulea grown in SMF medium containing 2- 13 C-glucose in a yield of about 13 g/100 g mycelia. © 2011 Elsevier Ltd. All rights reserved.